- Open Access
Decitabine impact on the endocytosis regulator RhoA, the folate carriers RFC1 and FOLR1, and the glucose transporter GLUT4 in human tumors
- David J Stewart1Email author,
- Maria I Nunez2,
- Jaroslav Jelinek3,
- David Hong2,
- Sanjay Gupta2,
- Jean-Pierre Issa†3,
- Ignacio I Wistuba†2 and
- Razelle Kurzrock†4
© Stewart et al.; licensee BioMed Central Ltd. 2014
- Received: 9 August 2013
- Accepted: 13 December 2013
- Published: 9 January 2014
In 31 solid tumor patients treated with the demethylating agent decitabine, we performed tumor biopsies before and after the first cycle of decitabine and used immunohistochemistry (IHC) to assess whether decitabine increased expression of various membrane transporters. Resistance to chemotherapy may arise due to promoter methylation/downregulation of expression of transporters required for drug uptake, and decitabine can reverse resistance in vitro. The endocytosis regulator RhoA, the folate carriers FOLR1 and RFC1, and the glucose transporter GLUT4 were assessed.
Pre-decitabine RhoA was higher in patients who had received their last therapy >3 months previously than in patients with more recent prior therapy (P = 0.02), and varied inversely with global DNA methylation as assessed by LINE1 methylation (r = −0.58, P = 0.006). Tumor RhoA scores increased with decitabine (P = 0.03), and RFC1 also increased in patients with pre-decitabine scores ≤150 (P = 0.004). Change in LINE1 methylation with decitabine did not correlate significantly with change in IHC scores for any transporter assessed. We also assessed methylation of the RFC1 gene (alias SLC19A1). SLC19A1 methylation correlated with tumor LINE1 methylation (r = 0.45, P = 0.02). There was a small (statistically insignificant) decrease in SLC19A1 methylation with decitabine, and there was a trend towards change in SLC19A1 methylation with decitabine correlating with change in LINE1 methylation (r = 0.47, P <0.15). While SLC19A1 methylation did not correlate with RFC1 scores, there was a trend towards an inverse correlation between change in SLC19A1 methylation and change in RFC1 expression (r = −0.45, P = 0.19).
In conclusion, after decitabine administration, there was increased expression of some (but not other) transporters that may play a role in chemotherapy uptake. Larger patient numbers will be needed to define the extent to which this increased expression is associated with changes in DNA methylation.
- LINE1 methylation
- Promoter methylation
Resistance to chemotherapy can arise from overexpression of resistance factors or from underexpression of factors required for drug efficacy [1, 2]. Dose–response curve flattening at higher chemotherapy doses suggests that incurability of epithelial malignancies may be due primarily to underexpression of factors required for cytotoxicity . If resistance were instead due to overexpression of resistance factors, one would expect a shoulder on a log-linear dose–response curve (with increasing efficacy at higher doses) instead of curve flattening at higher doses . Examples of factors required for cytotoxicity that may be deficient in resistant cells include drug uptake transporters (for example, CTR1 for platinums and folate transporters for pemetrexed), drug activating enzymes (for example, deoxycytidine kinase for gemcitabine), obligate drug targets (for example, topoisomerase II for etoposide and doxorubicin) and pro-apoptotic molecules [2, 4, 5].
Deficiency of factors required for drug uptake and activation might directly potentiate resistance by reducing the amount of active drug in a cell, but could also have a secondary effect, in that these transporters may also play a role in uptake and cellular metabolism of nutrients. Hence, deficiency in these factors could reduce the rate of tumor cell division, and quiescent cells are generally more resistant to chemotherapy than are actively dividing cells [1, 2, 4]. Decreased uptake of several agents  and downregulation of expression of various transporters including folate binding protein and the endocytosis regulator RhoA  has been described in cisplatin-resistant cells that also have a reduced cell growth rate.
The mechanism by which transporters and other factors required for drug efficacy may be downregulated in cancer cells remains unclear. However, promoter hypermethylation is one mechanism by which gene expression may be downregulated, and hence DNA methylation could play a role in underexpression of factors required for drug efficacy [8–18]. Cancer cells often have abnormal hypermethylation and silencing of tumor suppressor genes [8–11] and of genes that support chemotherapy cytotoxicity [12, 13]. Several genes may be hypermethylated in resistant cell lines [14, 15] or tumors .
If DNA hypermethylation might play a role in resistance, then it follows that agents that reduce DNA methylation might sensitize cells to chemotherapy. The DNA demethylating agent decitabine reversed folate binding protein downregulation in cisplatin-resistant cells , augmented cellular uptake of methotrexate and carboplatin , and reversed resistance to various chemotherapy [15–17, 19–24] or targeted agents  by upregulating expression of proapoptotic factors [19, 20, 25] and by other mechanisms . DNA methylation also protected the anti-apoptotic factor survivin from repression by p53, and decitabine reversed this, permitting survivin repression by p53 . Decitabine also partially reversed resistance to carboplatin in patients with advanced ovarian cancer [18, 27, 28].
The related DNA demethylating agent 5-azacytidine downregulated telomerase expression  and potentiated cisplatin [30–32], carboplatin , and docetaxel [31, 32, 34] preclinically by decreasing expression of pAKT [30, 31] and other anti-apoptotic factors , by increasing expression of the tumor suppressor gene TMS1 and various proapoptotic factors [31, 33], and by other mechanisms . Also, 5-Azacytidine potentiated irinotecan in p53-mutant cells by upregulating expression of its obligate target topoisomerase-I via mechanisms involving p16 demethylation and Sp1 upregulation .
Decitabine is active clinically in myelodysplasia and leukemia [36–38]. Low-dose administration days 1 to 5 +/− days 8 to 12 of a cycle may be most effective [36–39]. Low-dose regimens are also particularly likely to induce DNA demethylation [38, 39].
We administered low-dose decitabine days 1 to 5 +/− days 8 to 12 each cycle to patients with refractory malignancies and biopsied tumors before day 1 and on day 12 of cycle 1 . In that study, decitabine decreased methylation of the long interspersed nuclear element 1 (LINE1) repetitive element (which was used as a surrogate for ‘global’ tumor DNA methylation) and increased tumor expression of the copper/platinum transporter CTR1. Pre-decitabine tumor expression of CTR1 was lower and LINE1 methylation tended to be higher in patients who were ≤3 months versus >3 months beyond most recent prior therapy . Decitabine’s impact on CTR1 was greatest in patients more recently exposed to other agents. CTR1 expression correlated inversely with LINE1 methylation, although the CTR1 promoter was not methylated .
Based on our observations with CTR1 , we then asked whether other selected transporters behaved in a similar manner in these same tumor specimens. Specifically, we asked whether expression of other transporters is decreased in tumors of patients recently exposed to chemotherapy and targeted agents, whether tumor expression of transporters correlated inversely with LINE1 methylation, whether decitabine augmented transporter expression, and whether promoter methylation could explain any impact of decitabine on expression of one of these transporters.
Patient characteristics have been reported in detail previously . Tumor tissue was not available from all patients for all assessments, and hence patient numbers varied across assessments. Patient numbers varied slightly between different transporters since insufficient biopsy material was available for some assessments. Of the 31 patients treated on our decitabine trial , 27 had adequate tissue for at least one pre- or post-decitabine IHC assessment of one or more transporters. The 27 evaluable patients included 15 males and 12 females. Tumor types included cancers of the breast (four patients), kidney (three), head and neck (four, including two adenocystic carcinomas), lung (one), stomach (one), endometrium (one), and appendix (one), malignant melanomas (four), thymic neoplasms (three), neuroendocrine tumors (two), lymphomas (two), and desmoplastic tumor (one). Patients had received a median (range) of 5 (1 to 14) prior systemic regimens and a median (range) of 2 (0 to 6) prior targeted agents. Time from last prior treatment until entry unto this study varied substantially between patients. In patients with longer time intervals, this was primarily a function of relatively prolonged control or indolent tumor growth after discontinuation of their most recent prior therapy.
Transporter immunohistochemistry scores and tumor type
Compared to other tumor types, adenocarcinomas had higher pre-decitabine IHC scores for reduced folate carrier (RFC1) (median score 240 in adenocarcinomas versus 80 in others, P = 0.0096). Adenocarcinomas also had a higher median pre-decitabine score for folate receptor-alpha (FOLR1) (90 versus 60, P = 0.0146). There were no major differences between adenocarcinomas and other tumor types for pre-decitabine scores for the endocytosis regulator/small GTPase RhoA (median scores 50 for both adenocarcinomas and others, P = 0.7758) or for the glucose transporter GLUT4 (median score 22.5 for adenocarcinomas versus 10 for others, P = 0.52).
Transporter immunohistochemistry scores and time from last therapy
Transporter immunohistochemistry scores versus LINE1 methylation (as a surrogate for global DNA methylation)
Correlation of pre-decitabine immunohistochemistry (IHC) scores for transporters with percent LINE1 methylation
Decitabine effect on transporter immunohistochemistry scores
RFC1 scores increased in 14 of 21 evaluable patients with decitabine therapy, although the median score remained unchanged at 90 (P = 0.17). However, if only patients with low pre-decitabine scores (<150) were assessed, then RFC1 scores increased modestly in 13 of 17 patients (from a median score of 80 to a median score of 90, P = 0.004). FOLR1 and GLUT4 scores did not vary significantly with decitabine therapy. Change in IHC scores did not vary significantly with decitabine dose (data not shown).
Impact of decitabine on transporter IHC scores did not appear to vary with tumor type. The changes in median IHC scores with decitabine were 30 (adenocarcinomas) versus 20 (others) for RhoA (P = 0.63), 30 (adenocarcinomas) versus 12 (others) for RFC1, -5 (adenocarcinomas) versus 0 (others) for FOLR1 (P = 0.89) and 0 (adenocarcinomas) versus 0 (others) for GLUT4 (P = 0.96).
Post-decitabine changes in transporter immunohistochemistry scores versus changes in LINE1 methylation
Correlation of change in transporter immunohistochemistry (IHC) scores with change in percent LINE1 methylation
Decitabine effect on promoter methylation
Since IHC scores for RFC1 increased over the course of decitabine therapy in patients who initially had low scores, we assessed promoter methylation for its gene (alias SLC19A1) in patients with sufficient DNA. The SLC19A1 assay (designed at the edge of the CpG island − 700 base pairs from the transcription start site) showed median methylation of 64% (range, 21 to 83%) in pre-decitabine tumor samples, compared to 18% in patient blood samples, 10% in normal control adult blood samples, 9% in control umbilical cord blood samples, and 63% in leukemia cell lines. Median SLC19A1 methylation was 57.5% in post-decitabine tumor samples (range, 19-83%) (P = 0.63 in Wilcoxon signed rank test paired comparisons for 10 patients with both pre- and post-decitabine evaluable tumor samples). Changes in SLC19A1 methylation did not vary with decitabine dose (data not shown).
SLC19A1 methylation correlated with LINE1 methylation in 26 evaluable pre- and post-decitabine tumor samples (r = 0.45, P = 0.02), and there was a trend towards change in SLC19A1 methylation varying with change in tumor LINE1 methylation in patients (n = 11) for whom both pre- and post-decitabine tumor samples were evaluable for both genes (n = 11, r = 0.47, P = 0.1457). While RFC1 protein expression did not correlate with SLC19A1 methylation across all evaluable tumor samples (n = 24, r = −0.009, P = 0.97), there was a trend towards change in RFC1 protein expression varying inversely with change in SLC19A1 methylation for patients in who both pre- and post-decitabine tumor samples were evaluable (n = 10, r = −0.45, P = 0.1912).
In earlier studies, we found in patient tumor samples that IHC scores for the copper/platinum transporter CTR1 increased with increasing time from exposure to last prior therapy , and we interpreted this as possible evidence that prior therapy might induce cross-resistance to platinums by downregulating CTR1, although other explanations for our CTR1 observation are possible. (For example, long time interval from last prior therapy to decitabine might also have been due to indolence of the patient’s tumor, and it is possible that high CTR1 expression could be a marker of tumor indolence such that patients with high CTR1 tolerated longer time intervals off therapy. However, in our earlier paper we found that high CTR1 expression correlated with high mitotic count , making this explanation unlikely). In our earlier paper, we also noted that tumor CTR1 IHC scores correlated inversely with LINE1 methylation (a putative marker of global DNA methylation), and that tumor CTR1 IHC scores increased after therapy with the DNA demethylating agent decitabine . Liang et al. demonstrated a pleiotropic reduction in membrane transporters in platinum-resistant tumor cell lines , and Shen et al. reported that decitabine upregulated expression of a folate transporter and increased carboplatin and methotrexate uptake in one of these cell lines . Several investigators demonstrated that decitabine or the related DNA demethylating agent 5-azacytidine can reverse resistance to chemotherapy in tumor cell lines, or can induce changes that might reasonably be expected to reverse resistance [13, 19, 20, 25, 26, 29–31, 33–35].
Based on these different lines of evidence, we hypothesized that exposure to a wide range of chemotherapy drugs or targeted agents might generate broad cross-resistance by downregulating expression of a range of unrelated membrane transporters. We further hypothesized that this transporter downregulation could occur through promoter hypermethylation and might be reversible by demethylating agents. Our findings from the current study failed to confirm our hypothesis. While our findings with the endocytosis regulator RhoA were very similar to our previous observations with CTR1, the other transporters behaved differently. Like CTR1 , RhoA scores varied inversely with LINE1 methylation and increased with time from last therapy exposure and with decitabine treatment, and others have reported that RhoA gene hypermethylation was associated with reduced RhoA expression in human tumors . However, unlike CTR1  and RhoA, scores for the transporters RFC1, FOLR1 and GLUT4 did not increase with time from last drug exposure and did not vary inversely with LINE1 methylation, although RFC1 did increase modestly with decitabine. Hence, exposure to a wide range of agents could potentially lead to a limited spectrum of cross-resistance by downregulating expression of some specific transporters, and decitabine could potentially increase expression of some transporters, but not others. It remains untested whether decitabine can reverse resistance to agents that rely on these transporters for uptake into tumor cells.
On the other hand, while a broad downregulation of transporters was not noted in our study patients with recent prior therapy exposure, we cannot comment on transporter function, and there are other factors that could come into play in limiting drug uptake. Specifically, a mislocalization of membrane proteins due to defective plasma membrane protein recycling has been noted in cisplatin-resistant cell lines , and this in turn was linked to defective endocytosis and to down-regulation of small GTPases including RhoA . Hence, down-regulation of the small GTPases might potentially be sufficient to decrease uptake of a range of agents, and restoring RhoA and related factors could possibly reverse this, even if there is no obvious impact on expression of transporters.
The mechanism by which decitabine increased CTR1 scores (in our previous study ) and RhoA and RFC1 (in the present study) is not clear. Decitabine can increase expression of specific genes through mechanisms that are both dependent on  and independent of [9, 11] promoter hypermethylation. We found no evidence of CTR1 promoter hypermethylation in our previous study . In the current study, we did find promoter methylation of the RFC1 gene (alias SLC19A1), but there was only a modest reduction in SLC19A1 promoter methylation with decitabine. Furthermore, RFC1 scores did not correlate with SLC19A1 methylation, although there was a trend towards change in SLC19A1 methylation correlating with change in RFC1 score.
The reduced folate carrier is the major uptake mediator of anticancer antifolates and silencing of the reduced folate carrier gene (through a mechanism that appeared to be independent of promoter methylation) was noted in multiple resistant tumor cell lines . Exposure of cell lines to methotrexate downregulated expression of RFC1, and this was not prevented by 5-azacytidine . Since folic acid insufficiency alters DNA methylation , since there is an inverse relationship between folate levels and DNA methylation in human tumors , and since folic acid supplementation appears to induce DNA hypomethylation [46, 47] in some circumstances (possibly by decreasing production of S-adenosylmethionine, the methyl donor for DNA methyltransferase) , it would also be of interest to assess whether addition of folic acid augments the ability of decitabine to induce DNA hypomethylation and restore silenced gene function. If decitabine can increase uptake of folate into tumors by increasing RFC1 expression, then folic acid and decitabine could possibly potentiate each other’s effects.
Overall, our observations suggest that it would be reasonable to test decitabine clinically in combination with other agents (including antifolates and platinums) to determine if it can prevent or reverse resistance that arises due to reduced drug uptake, and the experience to date in platinum-resistant ovarian cancer is encouraging [18, 27, 28]. It might be particularly useful to test its ability to potentiate chemotherapy in patients with low baseline expression of RhoA, RFC1 and/or CTR1, in those with higher baseline LINE1 methylation, and/or in those with a shorter time interval since last prior therapy. As noted previously, there are also several other mechanisms by which demethylating agents may prevent or reverse resistance to a variety of agents [7, 13, 14, 19–21, 25, 26, 29–31, 33–35].
However, addition of decitabine to other agents could also have adverse consequences. For example, while we previously reported that decitabine therapy was associated with increased apoptosis in human tumors, we also found that mitoses and Ki-67 expression tended to increase with decitabine administration in tumors in which they were initially low  (suggesting that decitabine possibly might stimulate proliferation of quiescent tumor cells). While this might increase sensitivity of quiescent tumors to chemotherapy, it could also lead to resistance through accelerated repopulation. Furthermore, others have demonstrated that decitabine may reduce tumor cell sensitivity to cisplatin by reversing promoter-methylation-induced downregulation of the resistance factor glutathione-S-transferase-pi  and demethylating agents also increased tumor cell expression of resistance-associated drug efflux pumps, including MDR1/p-glycoprotein [49, 50]. While some studies have suggested that DNA demethylation may increase efficacy of topoisomerase-1 inhibitors , others suggested that decitabine-induced DNA hypomethylation reduced camptothecin’s ability to induce DNA strand breaks . The related agent 5-azacytidine augmented expression of the DNA repair enzyme ERCC1 by reducing its promoter methylation, and thereby decreased tumor cell sensitivity to radiation . Tumor cell expression of metallothioneins (which may be important in chemotherapy resistance ) was also reduced by promoter methylation and augmented by 5-azacytidine .
In summary, decitabine administration was associated with biological changes in human tumors that could prove therapeutically useful, particularly if decitabine is combined with other agents. However, as we have argued previously for anticancer therapies in general , the broad range of decitabine’s potential effects mean that in future studies we should use extensive molecular characterization of patients’ tumors to carefully assess which patients are most likely to be helped versus harmed by decitabine use.
Patients and methods
As previously reported , eligibility criteria for this study (approved by the University of Texas MD Anderson Cancer Center Research Ethics Board) included written, informed consent, diagnosis of incurable malignancy refractory to standard therapy, adequate organ function, and tumor amenable to biopsy. Decitabine (supplied under a Collaborative and Research Development Agreement by the National Cancer Institute Division of Cancer Treatment and Diagnosis) was administered intravenously over one hour . Doses were 2.5, 5, or 10 mg/m2/day on days 1 to 5 and 8 to 12 each 4-week cycle or 15 or 20 mg/m2/day on days 1 to 5 each cycle. Filgrastim was added at higher doses.
Antibodies used for immunohistochemistry
Polyclonal (antibody 62302)
Abcam, Cambridge, MA, USA
Monoclonal IgG, clone Mb343
Homemade, kindly supplied by Dr. Wilbur Franklin 
Abcam, Cambridge, MA, USA
Novus Biologicals, Littleton, CO, USA
Primers used for pyrosequencing assessments of SLC19A1 methylation
Non-parametric two-tailed statistics were calculated using GraphPad Prism 5.0 (Spearman tests for correlations, Wilcoxon signed rank tests for paired comparisons, and Mann–Whitney tests for comparisons of two groups). Low patient numbers limited statistical power.
Supported in part by NIH grants UO1 CA062461-10, R21 CA112895-01A1, Cancer Center Support Grant number 5-P30 CA16672-32, and by Department of Defense grant number W81XWH-07-1-0306. We would like to thank MD Anderson Cancer Center for making funding available for required tumor biopsies and would also like to express our appreciation for the help and support for this project that we received from the late Dr. Igor Espinoza-Delgado while he was at CTEP, NCI. Presented in part at the 100th annual meeting of the American Association for Cancer Research, Denver, CO, April 18 to 22, 2009 and the 45th annual meeting of the American Society of Clinical Oncology, Orlando, FL, May 29-June 2, 2009.
- Stewart DJ, Raaphorst GP, Yau J, Beaubien AR: Active vs. passive resistance, dose–response relationships, high dose chemotherapy, and resistance modulation: a hypothesis. Invest New Drugs. 1996, 14: 115-130.PubMedGoogle Scholar
- Stewart DJ: Tumor and host factors that may limit efficacy of chemotherapy in non-small cell and small cell lung cancer. Crit Rev Oncol Hematol. 2010, 75: 173-234. 10.1016/j.critrevonc.2009.11.006.PubMed CentralView ArticlePubMedGoogle Scholar
- Stewart DJ, Chiritescu G, Dahrouge S, Banerjee S, Tomiak EM: Chemotherapy dose–response relationships in non-small cell lung cancer and implied resistance mechanisms. Cancer Treat Rev. 2007, 33: 101-137. 10.1016/j.ctrv.2006.12.002.View ArticlePubMedGoogle Scholar
- Stewart DJ: Mechanisms of resistance to cisplatin and carboplatin. Crit Rev Oncol Hematol. 2007, 63: 12-31. 10.1016/j.critrevonc.2007.02.001.View ArticlePubMedGoogle Scholar
- Zhao R, Qiu A, Tsai E, Jansen M, Akabas MH, Goldman ID: The proton-coupled folate transporter: impact on pemetrexed transport and on antifolates activities compared with the reduced folate carrier. Mol Pharmacol. 2008, 74: 854-862. 10.1124/mol.108.045443.PubMed CentralView ArticlePubMedGoogle Scholar
- Liang XJ, Shen DW, Gottesman MM: A pleiotropic defect reducing drug accumulation in cisplatin-resistant cells. J Inorg Biochem. 2004, 98: 1599-1606. 10.1016/j.jinorgbio.2004.05.015.View ArticlePubMedGoogle Scholar
- Shen DW, Su A, Liang XJ, Pai-Panandiker A, Gottesman MM: Reduced expression of small GTPases and hypermethylation of the folate binding protein gene in cisplatin-resistant cells. Br J Cancer. 2004, 91: 270-276.PubMed CentralPubMedGoogle Scholar
- Kim JS, Kim H, Shim YM, Han J, Park J, Kim DH: Aberrant methylation of the FHIT gene in chronic smokers with early stage squamous cell carcinoma of the lung. Carcinogenesis. 2004, 25: 2165-2171. 10.1093/carcin/bgh217.View ArticlePubMedGoogle Scholar
- Oki Y, Aoki E, Issa JP: Decitabine–bedside to bench. Crit Rev Oncol Hematol. 2007, 61: 140-152. 10.1016/j.critrevonc.2006.07.010.View ArticlePubMedGoogle Scholar
- Baylin SB, Herman JG, Graff JR, Vertino PM, Issa JP: Alterations in DNA methylation: a fundamental aspect of neoplasia. Adv Cancer Res. 1998, 72: 141-196.View ArticlePubMedGoogle Scholar
- Ghoshal K, Bai S: DNA methyltransferases as targets for cancer therapy. Drugs Today (Barc). 2007, 43: 395-422. 10.1358/dot.2007.43.6.1062666.View ArticleGoogle Scholar
- Chekhun VF, Lukyanova NY, Kovalchuk O, Tryndyak VP, Pogribny IP: Epigenetic profiling of multidrug-resistant human MCF-7 breast adenocarcinoma cells reveals novel hyper- and hypomethylated targets. Mol Cancer Ther. 2007, 6: 1089-1098. 10.1158/1535-7163.MCT-06-0663.View ArticlePubMedGoogle Scholar
- Plumb JA, Strathdee G, Sludden J, Kaye SB, Brown R: Reversal of drug resistance in human tumor xenografts by 2'-deoxy-5-azacytidine-induced demethylation of the hMLH1 gene promoter. Cancer Res. 2000, 60: 6039-6044.PubMedGoogle Scholar
- Chang X, Monitto CL, Demokan S, Kim MS, Chang SS, Zhong X, Califano JA, Sidransky D: Identification of hypermethylated genes associated with cisplatin resistance in human cancers. Cancer Res. 2010, 70: 2870-2879. 10.1158/0008-5472.CAN-09-3427.PubMed CentralView ArticlePubMedGoogle Scholar
- Zeller C, Dai W, Steele NL, Siddiq A, Walley AJ, Wilhelm-Benartzi CS, Rizzo S, van der Zee A, Plumb JA, Brown R: Candidate DNA methylation drivers of acquired cisplatin resistance in ovarian cancer identified by methylome and expression profiling. Oncogene. 2012, 31: 4567-4576. 10.1038/onc.2011.611.View ArticlePubMedGoogle Scholar
- Tanaka T, Bai T, Toujima S, Utsunomiya T, Matsuoka T, Kobayashi A, Yamamoto M, Sasaki N, Tanizaki Y, Utsunomiya H, Tanaka J, Yukawa K: Demethylation restores SN38 sensitivity in cells with acquired resistance to SN38 derived from human cervical squamous cancer cells. Oncol Rep. 2012, 27: 1292-1298.PubMed CentralPubMedGoogle Scholar
- Vijayaraghavalu S, Peetla C, Lu S, Labhasetwar V: Epigenetic modulation of the biophysical properties of drug-resistant cell lipids to restore drug transport and endocytic functions. Mol Pharm. 2012, 9: 2730-2742. 10.1021/mp300281t.PubMed CentralView ArticlePubMedGoogle Scholar
- Matei D, Fang F, Shen C, Schilder J, Arnold A, Zeng Y, Berry WA, Huang T, Nephew KP: Epigenetic resensitization to platinum in ovarian cancer. Cancer Res. 2012, 72: 2197-2205. 10.1158/0008-5472.CAN-11-3909.PubMed CentralView ArticlePubMedGoogle Scholar
- Muerkoster SS, Werbing V, Koch D, Sipos B, Ammerpohl O, Kalthoff H, Tsao MS, Folsch UR, Schafer H: Role of myofibroblasts in innate chemoresistance of pancreatic carcinoma–epigenetic downregulation of caspases. Int J Cancer. 2008, 123: 1751-1760. 10.1002/ijc.23703.View ArticlePubMedGoogle Scholar
- Shang D, Liu Y, Liu Q, Zhang F, Feng L, Lv W, Tian Y: Synergy of 5-aza-2'-deoxycytidine (DAC) and paclitaxel in both androgen-dependent and -independent prostate cancer cell lines. Cancer Lett. 2009, 278: 82-87. 10.1016/j.canlet.2008.12.034.View ArticlePubMedGoogle Scholar
- Ding L, Qiu L, Zhang J, Guo B: Camptothecin-induced cell proliferation inhibition and apoptosis enhanced by DNA methyltransferase inhibitor, 5-aza-2'-deoxycytidine. Biol Pharm Bull. 2009, 32: 1105-1108. 10.1248/bpb.32.1105.View ArticlePubMedGoogle Scholar
- Charlet J, Schnekenburger M, Brown KW, Diederich M: DNA demethylation increases sensitivity of neuroblastoma cells to chemotherapeutic drugs. Biochem Pharmacol. 2012, 83: 858-865. 10.1016/j.bcp.2012.01.009.View ArticlePubMedGoogle Scholar
- Iwata H, Sato H, Suzuki R, Yamada R, Ichinomiya S, Yanagihara M, Okabe H, Sekine Y, Yano T, Ueno K: A demethylating agent enhances chemosensitivity to vinblastine in a xenograft model of renal cell carcinoma. Int J Oncol. 2011, 38: 1653-1661.PubMedGoogle Scholar
- Onda K, Suzuki R, Tanaka S, Oga H, Oka K, Hirano T: Decitabine, a DNA methyltransferase inhibitor, reduces P-glycoprotein mRNA and protein expressions and increases drug sensitivity in drug-resistant MOLT4 and Jurkat cell lines. Anticancer Res. 2012, 32: 4439-4444.PubMedGoogle Scholar
- San Jose-Eneriz E, Agirre X, Jimenez-Velasco A, Cordeu L, Martin V, Arqueros V, Garate L, Fresquet V, Cervantes F, Martinez-Climent JA, Heiniger A, Torres A, Prósper F, Roman-Gomez J: Epigenetic down-regulation of BIM expression is associated with reduced optimal responses to imatinib treatment in chronic myeloid leukaemia. Eur J Cancer. 2009, 45: 1877-1889. 10.1016/j.ejca.2009.04.005.View ArticlePubMedGoogle Scholar
- Nabilsi NH, Broaddus RR, Loose DS: DNA methylation inhibits p53-mediated survivin repression. Oncogene. 2009, 28: 2046-2050. 10.1038/onc.2009.62.View ArticlePubMedGoogle Scholar
- Fu S, Hu W, Iyer R, Kavanagh JJ, Coleman RL, Levenback CF, Sood AK, Wolf JK, Gershenson DM, Markman M, Hennessy BT, Kurzrock R, Bast RC: Phase 1b-2a study to reverse platinum resistance through use of a hypomethylating agent, azacitidine, in patients with platinum-resistant or platinum-refractory epithelial ovarian cancer. Cancer. 2011, 117: 1661-1669. 10.1002/cncr.25701.PubMed CentralView ArticlePubMedGoogle Scholar
- Fang F, Balch C, Schilder J, Breen T, Zhang S, Shen C, Li L, Kulesavage C, Snyder AJ, Nephew KP, Matei DE: A phase 1 and pharmacodynamic study of decitabine in combination with carboplatin in patients with recurrent, platinum-resistant, epithelial ovarian cancer. Cancer. 2010, 116: 4043-4053. 10.1002/cncr.25204.PubMed CentralView ArticlePubMedGoogle Scholar
- Kumari A, Srinivasan R, Wig JD: Effect of c-MYC and E2F1 gene silencing and of 5-azacytidine treatment on telomerase activity in pancreatic cancer-derived cell lines. Pancreatology. 2009, 9: 360-368. 10.1159/000212094.View ArticlePubMedGoogle Scholar
- Tikoo K, Ali IY, Gupta J, Gupta C: 5-Azacytidine prevents cisplatin induced nephrotoxicity and potentiates anticancer activity of cisplatin by involving inhibition of metallothionein, pAKT and DNMT1 expression in chemical induced cancer rats. Toxicol Lett. 2009, 191: 158-166. 10.1016/j.toxlet.2009.08.018.View ArticlePubMedGoogle Scholar
- Festuccia C, Gravina GL, D'Alessandro AM, Muzi P, Millimaggi D, Dolo V, Ricevuto E, Vicentini C, Bologna M: Azacitidine improves antitumor effects of docetaxel and cisplatin in aggressive prostate cancer models. Endocr Relat Cancer. 2009, 16: 401-413. 10.1677/ERC-08-0130.View ArticlePubMedGoogle Scholar
- Ramachandran K, Gordian E, Singal R: 5-azacytidine reverses drug resistance in bladder cancer cells. Anticancer Res. 2011, 31: 3757-3766.PubMedGoogle Scholar
- Li Y, Hu W, Shen DY, Kavanagh JJ, Fu S: Azacitidine enhances sensitivity of platinum-resistant ovarian cancer cells to carboplatin through induction of apoptosis. Am J Obstet Gynecol. 2009, 200: 177-e171-e179PubMedGoogle Scholar
- Gordian E, Ramachandran K, Singal R: Methylation mediated silencing of TMS1 in breast cancer and its potential contribution to docetaxel cytotoxicity. Anticancer Res. 2009, 29: 3207-3210.PubMedGoogle Scholar
- Crea F, Giovannetti E, Cortesi F, Mey V, Nannizzi S, Gallegos Ruiz MI, Ricciardi S, Del Tacca M, Peters GJ, Danesi R: Epigenetic mechanisms of irinotecan sensitivity in colorectal cancer cell lines. Mol Cancer Ther. 2009, 8: 1964-1973. 10.1158/1535-7163.MCT-09-0027.View ArticlePubMedGoogle Scholar
- Kantarjian HM, O'Brien S, Huang X, Garcia-Manero G, Ravandi F, Cortes J, Shan J, Davisson J, Bueso-Ramos CE, Issa JP: Survival advantage with decitabine versus intensive chemotherapy in patients with higher risk myelodysplastic syndrome: comparison with historical experience. Cancer. 2007, 109: 1133-1137. 10.1002/cncr.22508.PubMed CentralView ArticlePubMedGoogle Scholar
- Issa JP, Garcia-Manero G, Giles FJ, Mannari R, Thomas D, Faderl S, Bayar E, Lyons J, Rosenfeld CS, Cortes J, Kantarjian HM: Phase 1 study of low-dose prolonged exposure schedules of the hypomethylating agent 5-aza-2'-deoxycytidine (decitabine) in hematopoietic malignancies. Blood. 2004, 103: 1635-1640. 10.1182/blood-2003-03-0687.View ArticlePubMedGoogle Scholar
- Issa JP, Gharibyan V, Cortes J, Jelinek J, Morris G, Verstovsek S, Talpaz M, Garcia-Manero G, Kantarjian HM: Phase II study of low-dose decitabine in patients with chronic myelogenous leukemia resistant to imatinib mesylate. J Clin Oncol. 2005, 23: 3948-3956. 10.1200/JCO.2005.11.981.View ArticlePubMedGoogle Scholar
- Kantarjian H, Oki Y, Garcia-Manero G, Huang X, O'Brien S, Cortes J, Faderl S, Bueso-Ramos C, Ravandi F, Estrov Z, Ferrajoli A, Wierda W, Shan J, Davis J, Giles F, Saba HI, Issa JP: Results of a randomized study of 3 schedules of low-dose decitabine in higher-risk myelodysplastic syndrome and chronic myelomonocytic leukemia. Blood. 2007, 109: 52-57. 10.1182/blood-2006-05-021162.View ArticlePubMedGoogle Scholar
- Stewart DJ, Issa JP, Kurzrock R, Nunez MI, Jelinek J, Hong D, Oki Y, Guo Z, Gupta S, Wistuba II: Decitabine effect on tumor global DNA methylation and other parameters in a phase I trial in refractory solid tumors and lymphomas. Clin Cancer Res. 2009, 15: 3881-3888. 10.1158/1078-0432.CCR-08-2196.View ArticlePubMedGoogle Scholar
- Braga EA, Loginov VI, Klimov EA, Kilosanidze G, Khodyrev DS, Kaganova NL, Kazybskaia TP, Ermilova VD, Gar'kavtseva RF, Pronina IV, Rud'ko OI, Zabarskiĭ ER, Sulimova GE, Kiselev LL: [Activation of RHOA gene transcription in epithelial tumors may be caused by gene amplification and/or demethylation of its promotor region]. Mol Biol (Mosk). 2006, 40: 865-877.View ArticleGoogle Scholar
- Rothem L, Stark M, Kaufman Y, Mayo L, Assaraf YG: Reduced folate carrier gene silencing in multiple antifolate-resistant tumor cell lines is due to a simultaneous loss of function of multiple transcription factors but not promoter methylation. J Biol Chem. 2004, 279: 374-384.View ArticlePubMedGoogle Scholar
- Ma D, Huang H, Moscow JA: Down-regulation of reduced folate carrier gene (RFC1) expression after exposure to methotrexate in ZR-75-1 breast cancer cells. Biochem Biophys Res Comm. 2000, 279: 891-897. 10.1006/bbrc.2000.4019.View ArticlePubMedGoogle Scholar
- Kane MA: The role of folates in squamous cell carcinoma of the head and neck. Cancer Detect Prev. 2005, 29: 46-53. 10.1016/j.cdp.2004.08.002.View ArticlePubMedGoogle Scholar
- Piyathilake CJ, Johanning GL, Macaluso M, Whiteside M, Oelschlager DK, Heimburger DC, Grizzle WE: Localized folate and vitamin B-12 deficiency in squamous cell lung cancer is associated with global DNA hypomethylation. Nutr Cancer. 2000, 37: 99-107. 10.1207/S15327914NC3701_13.View ArticlePubMedGoogle Scholar
- Stidley CA, Picchi MA, Leng S, Willink R, Crowell RE, Flores KG, Kang H, Byers T, Gilliland FD, Belinsky SA: Multivitamins, folate, and green vegetables protect against gene promoter methylation in the aerodigestive tract of smokers. Cancer Res. 2010, 70: 568-574. 10.1158/0008-5472.CAN-09-3410.PubMed CentralView ArticlePubMedGoogle Scholar
- Finnell RH, Spiegelstein O, Wlodarczyk B, Triplett A, Pogribny IP, Melnyk S, James JS: DNA methylation in Folbp1 knockout mice supplemented with folic acid during gestation. J Nutr. 2002, 132 (8 Suppl): 2457S-2461S.PubMedGoogle Scholar
- Zhang Y, Qu X, Jing W, Hu X, Yang X, Hou K, Teng Y, Zhang J, Liu Y: GSTP1 determines cis-platinum cytotoxicity in gastric adenocarcinoma MGC803 cells: regulation by promoter methylation and extracellular regulated kinase signaling. Anticancer Drugs. 2009, 20: 208-214. 10.1097/CAD.0b013e328322fbaa.View ArticlePubMedGoogle Scholar
- Lee TB, Park JH, Min YD, Kim KJ, Choi CH: Epigenetic mechanisms involved in differential MDR1 mRNA expression between gastric and colon cancer cell lines and rationales for clinical chemotherapy. BMC Gastroenterol. 2008, 8: 33-10.1186/1471-230X-8-33.PubMed CentralView ArticlePubMedGoogle Scholar
- Reed K, Hembruff SL, Sprowl JA, Parissenti AM: The temporal relationship between ABCB1 promoter hypomethylation, ABCB1 expression and acquisition of drug resistance. Pharmacogenomics J. 10: 489-504.Google Scholar
- Orta ML, Mateos S, Cortes F: DNA demethylation protects from cleavable complex stabilization and DNA strand breakage induced by the topoisomerase type I inhibitor camptothecin. Mutagenesis. 2009, 24: 237-244. 10.1093/mutage/gep002.View ArticlePubMedGoogle Scholar
- Liu ZG, Chen HY, Cheng JJ, Chen ZP, Li XN, Xia YF: Relationship between methylation status of ERCC1 promoter and radiosensitivity in glioma cell lines. Cell Biol Int. 2009, 33: 1111-1117. 10.1016/j.cellbi.2009.07.004.View ArticlePubMedGoogle Scholar
- Tse KY, Liu VW, Chan DW, Chiu PM, Tam KF, Chan KK, Liao XY, Cheung AN, Ngan HY: Epigenetic alteration of the metallothionein 1E gene in human endometrial carcinomas. Tumour Biol. 2009, 30: 93-99. 10.1159/000218032.View ArticlePubMedGoogle Scholar
- Stewart DJ, Kurzrock R: Cancer: the road to Amiens. J Clin Oncol. 2009, 27: 328-333.View ArticlePubMedGoogle Scholar
- Shia J, Klimstra DS, Nitzkorski JR, Low PS, Gonen M, Landmann R, Weiser MR, Franklin WA, Prendergast FG, Murphy L, Tang LH, Temple L, Guillem JG, Wong WD, Paty PB: Immunohistochemical expression of folate receptor alpha in colorectal carcinoma: patterns and biological significance. Hum Pathol. 2008, 39: 498-505. 10.1016/j.humpath.2007.09.013.View ArticlePubMedGoogle Scholar
- Yang AS, Estecio MR, Doshi K, Kondo Y, Tajara EH, Issa JP: A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements. Nucleic Acids Res. 2004, 32: e38-10.1093/nar/gnh032.PubMed CentralView ArticlePubMedGoogle Scholar
- Kroeger H, Jelinek J, Estecio MR, He R, Kondo K, Chung W, Zhang L, Shen L, Kantarjian HM, Bueso-Ramos CE, Issa JP: Aberrant CpG island methylation in acute myeloid leukemia is accentuated at relapse. Blood. 2008, 112: 1366-1373. 10.1182/blood-2007-11-126227.PubMed CentralView ArticlePubMedGoogle Scholar
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