Figure 1

Association of time from last prior treatment to tumor biopsy with pre-decitabine immunohistochemistry (IHC) scores. Tumor expression (by IHC) for the small GTPase endocytosis regulator RhoA was higher with longer time intervals between prior therapy and biopsy (a, n = 18, median IHC score = 100 for patients with last prior therapy 4 to 18 months earlier versus 30 for patients with last prior therapy 1 to 3 months earlier, P = 0.02). Time from last prior therapy until pre-decitabine biopsy did not have a significant impact on tumor expression of the other transporters. For the folate transporter RFC1 (b, n = 19), the median score was 42.5 for patients with 4 to 18 months between prior therapy and biopsy versus 135 for those with a 1 to 3 month interval (P = 0.06). For the folate transporter FOLR1 (c, n = 19), median scores were 80 for both groups (P = 0.90). For the glucose transporter GLUT4 (d, n = 19), the median score was 15 for patients with 4 to 18 months between prior therapy and biopsy versus 10 for those with a 1 to 3 month interval (P = 0.97).