Table 4 Primers used for pyrosequencing assessments of SLC19A1 methylation
Step | Name | Sequence |
|---|---|---|
PCR1 | RFC1F1 | AGGGATAAGTATAGTTTGTTTTTGGGGAT |
PCR1 | RFC1R | AATAACCCAAACCCCCCTTCC |
PCR1 | RFC1RU | GGGACACCGCTGATCGTTTAATAACCCAAACCCCCCTTCC |
PCR2 | RFC1F2 | GTGATTAGTAAGGGTTTGTATTAAGGAGTAAG |
PCR2 | BioUni | [Biotin]GGGACACCGCTGATCGTTTA |
PSQ | RFC1S | TAGTTTTTATTTTAGTAGGGATAG |