Epigenetic mechanisms in virus-induced tumorigenesis
© Springer-Verlag 2011
Received: 21 November 2010
Accepted: 28 February 2011
Published: 23 March 2011
About 15–20% of human cancers worldwide have viral etiology. Emerging data clearly indicate that several human DNA and RNA viruses, such as human papillomavirus, Epstein–Barr virus, Kaposi’s sarcoma-associated herpesvirus, hepatitis B virus, hepatitis C virus, and human T-cell lymphotropic virus, contribute to cancer development. Human tumor-associated viruses have evolved multiple molecular mechanisms to disrupt specific cellular pathways to facilitate aberrant replication. Although oncogenic viruses belong to different families, their strategies in human cancer development show many similarities and involve viral-encoded oncoproteins targeting the key cellular proteins that regulate cell growth. Recent studies show that virus and host interactions also occur at the epigenetic level. In this review, we summarize the published information related to the interactions between viral proteins and epigenetic machinery which lead to alterations in the epigenetic landscape of the cell contributing to carcinogenesis.
KeywordsEpigenetics DNA methylation Histone modification Oncogenetic virus Human cancer
Viruses and cancer
Cancer research over the past five decades has revealed important role of viral infections in human cancer. Viral etiology of human neoplasms was first discovered at the turn of the nineteenth century, when Ciuffo and co-workers demonstrated that human warts can be transmitted by cell-free filtrates derived from lesions (Ciuffo 1907). Several years later, in 1911, P. Rous identified the first animal tumorigenic virus (Rous sarcoma virus) that induces development of spindle cell sarcoma in birds. The first human tumor-associated virus was discovered more recently, in 1964, by Michael Anthony Epstein and Yvonne Barr and was named Epstein–Barr virus (EBV).
Recent studies have shown the infectious etiology of several cancers. It has been estimated that 15–20% of all human cancers worldwide are caused by oncogenic viruses (Butel 2000). Viruses associated with cancer belong to different phylogenetic groups. They include both DNA viruses, e.g., human papillomaviruses (HPV), hepatitis B virus (HBV), the herpesviruses such as EBV and Kaposi’s sarcoma-associated herpesvirus (KSHV), and RNA viruses such as retroviruses, e.g., human T-cell lymphotropic virus 1 (HTLV-1), and the RNA flavivirus, hepatitis C virus (HCV). The causal contribution of the above-mentioned viruses to the development of human neoplasms is now well documented. Besides these, there are other viruses with a potential influence on human carcinogenesis. Recently, an integrated form of a new polyomavirus, MCPyV, has been detected in patients with the Merkel cell carcinoma (zur Hausen 2008; Feng et al. 2008). Other polyomaviruses, such as SV40, JCV, and BKV, and adenoviruses may play possible role in human carcinogenesis as well. Polyomaviruses are tumorigenic under experimental conditions, and their genomic sequences were detected in samples derived from several human cancers, e.g., human osteosarcoma, mesotelioma, brain tumors, prostate cancer, and NHL; however, no definite proof exists that these viruses directly contribute to human cancer (McCabe et al. 2006; Goel et al. 2006; Feng et al. 2008; Jiang et al. 2009). Certain serotypes of adenoviruses are also highly transforming in cell culture and in animal models but adenovirus DNA was generally not detected in human tumor cells. However, one study reported detection of adenovirus DNA in pediatric brain tumors (Kosulin et al. 2007), therefore the possible contribution of adenoviruses to human oncogenesis should be considered.
Viruses associated with human cancer development
Cervical cancer, Anal cancer, Penis cancer, Head and neck carcinoma
Burkitt’s lymphoma, Hodgkin’s lymphoma, Posttransplantation lymphoma, Nasopharyngeal carcinoma
Kaposi’s sarcoma, Pleural effusion lymphoma, Multicentric Castleman’s disease
Adult T-cell leukemia
Merkel cell carcinoma
Mesothelioma and colon tumors
Brain and colon tumors
Prostate and brain tumors
Several serotypes can transform human and rodent cells and cause malignant tumors upon injection into rodents
Unlike acute-transforming animal retroviruses, human oncoviruses lead to cancer development with prolonged persistent infections. Additional factors such as environmental carcinogens, host cell mutations, and immune response also take part in viral-associated carcinogenesis. Viral strategies in human cancer development are diverse, depending on virus species and cell type they affect. Despite this, they share many common features. All human tumor-associated viruses encode oncoproteins essential for viral replication that disrupt cellular processes, such as apoptosis and cell-cycle checkpoint control (Butel 2000; McLaughlin-Drubin and Munger 2008). The main cellular targets of viral oncoproteins are p53 and RB, although recent studies also report other targets like nuclear factor κB (NFκB), hTERT, and TRAFs (Oliveira 2007; O’Shea 2005). The oncoproteins play very important role in viral life cycle. Because DNA oncoviruses rely on the cellular DNA replication machinery for propagation and most of them infect quiescent cells, which are not optimal for viral DNA replication, they evolved oncoproteins targeting the central cellular hubs regulating cell growth. This mechanism enables oncoviruses to force quiescent cells into unscheduled S-phase entry thus leading to concomitant DNA viral genome replication with host DNA. Deregulation of apoptosis and cell-cycle checkpoint control induced by tumorigenic viruses subsequently leads to an increase in cellular DNA mutations and genome instability (Butel 2000; Oliveira 2007; O’Shea 2005).
Recent cancer research provides the emerging information on the molecular events underlying the tumorigenic potential of human oncoviruses. During last two decades, significant progress has been made towards understanding the viral oncogenetic mechanisms. It has been demonstrated that the virus/host interactions that contribute to cancer development also occur at the epigenetic level.
The epigenetic state of cancer cell differs significantly from that of the normal cell. Cancer cells are characterized by multiple epigenetic alterations including DNA methylation and histone modification. Compared to the normal cells, cancer cells contain hypermethylated CpG islands in promoters of specific sets of genes and genome-wide hypomethylated DNA mainly in the body of genes and intergenic regions (Suzuki and Bird 2008; Esteller 2007; Kulis and Esteller 2010). The analysis of cancer cells epigenomes also revealed numerous aberrations in histone modifications including histone acetylation and methylation. These epigenetic aberrations lead to inappropriate gene expression that contributes to cancer development (Esteller 2007; Chi et al. 2010; Biancotto et al. 2010).
Increasing evidence reveals that oncogenic viruses also contribute to the epigenetic changes that are characteristic for cancer cells. Tumor-associated viruses interfere with host epigenetic machinery and cause aberrations of DNA methylation as well as changes in histone modifications. Many studies have shown that viral oncoproteins induce expression and interact with cellular DNA methyltransferases (DNMTs) as well as histone-modifying enzymes, e.g., histone deacetylases (HDACs), histone acetyltransferases (HATs), histone methyltransferases, and demethylases changing their activity (Burgers et al. 2007; Ferrari et al. 2009; McLaughlin-Drubin et al. 2011). Viral proteins are also able to alter the activity of proteins associated with the chromatin-remodeling complexes and miRNA processing (Flanagan 2007; Javier and Butel 2008; Whitby 2009).
Viruses that are able to integrate their genomes into host DNA often activate host defense mechanism that is responsible for the inactivation of integrated foreign genetic material by DNA methylation (Doerfler 1991a; 1996; 2009). Moreover, viral DNA methylation can be a masking mechanism that helps to avoid viral proteins recognition by the immune system during latent infections (Fernandez et al. 2009).
This review summarizes the information available about the epigenetic mechanisms used by human oncogenic viruses in human tumorigenesis. We describe the interactions between viral proteins and host epigenetic machinery and their consequences for the host cell epigenome and the viral life cycle.
HPV are small non-enveloped DNA viruses which infect epithelial cells and their life cycle depends on epithelial differentiation and viral–host protein interaction (Doorbar 2005, 2006). More than 100 different types of HPV have been identified and classified into low- or high-risk groups depending on their likelihood of inducing cervical cancer (zur Hausen 2009). Cervical cancer is one of the most common cancer among women worldwide and is strongly linked to infection by high-risk human papillomaviruses, mainly HPV16 and HPV18 types (de Villiers et al. 2004; zur Hausen 2009).
HPV E6 and E7 early proteins are the major HPV oncogenic proteins, which induce proliferation, immortalization, and malignant transformation of the infected cells. The key event in cervical carcinogenesis is integration of HPV genome into the host cell chromosome. In this case, virus is not able to complete its productive life cycle, and viruses are not released from infected cells, but can persist in the host cells and initiate oncogenesis. The integration frequently disrupts the E1-E2 genome region, resulting in a loss of E2 viral gene expression. E2 protein is a transcriptional repressor of E6 and E7 gene expression. Therefore, deregulation of E2 expression leads to an increase in expression of both E6 and E7 oncoproteins. Interactions between high-risk HPV16 E6 and E7 proteins and human tumor suppressor gene products p53 and retinoblastoma (RB), respectively, lead to functional inactivation of these critical cell regulatory proteins and thus contribute to tumorigenesis process.
Epigenetic alterations such as changes in DNA methylation pattern of viral and host genome as well as histone modification are very often associated with HPV infection and cervical carcinogenesis. Methylation of HPV DNA takes place regularly in vivo in cervical cells, clinical samples as well as in cell cultures. (Badal et al. 2003, 2004; Kim et al. 2003; van Tine et al. 2004; Kalantari et al. 2004; Wiley et al. 2005; Turan et al. 2006; Bhattacharjee and Sengupta 2006). It has been suggested that de novo methylation of HPV DNA might be a host defense mechanism for silencing viral replication and transcription or strategy that virus uses to maintain a long-term infection (Remus et al. 1999; Badal et al. 2003). HPV genome does not encode any known protein involved in DNA methylation machinery, therefore it is believed that the viral genome as well as the host genome is methylated by human host cell DNMT (Fernandez and Esteller 2010). The pattern of HPV genome methylation changes and depends on the stage of viral life cycle, and the presence of disease and probably the viral type (Woodman et al. 2007). Viral DNA hypermethylation is more closely associated with carcinomas than with asymptomatic infections or dysplasia (Fernandez et al. 2009). DNA hypermethylation has been observed in long control region (LCR) and L1 region of HPV genome (Badal et al. 2003, 2004; Kim et al. 2003; Kalantari et al. 2004; Bhattacharjee and Sengupta 2006; Turan et al. 2007; Hublarova et al. 2009). In the case of HPV16, LCR has been observed to be methylated in some primary cervical carcinomas, especially at E2-binding sites (E2BS; Bhattacharjee and Sengupta 2006; Brandsma et al. 2009; Fernandez et al. 2009). It has been proved in vitro that DNA methylation of the E2BS sequence inhibits the binding of E2 protein (Thain et al. 1996) and that this methylation is related to the reactivation of E6 and E7 in advanced stages of carcinogenesis induced by HPV16. It has been demonstrated that the use of DNA demethylating agents can induce recruitment of E2 protein to its upstream regulatory region-binding sites and reduce E6 and E7 expression (Fernandez et al. 2009). In the case of HPV18, LCR has been found to be methylated in several primary cell carcinomas and also in immortal descendant cells from primary human foreskin keratinocytes transfected with the entire HPV18 genome. However, the methylation of LCR has not been found in C41 and HeLa cell lines and the level of E6 and E7 was not modified by the treatment with DNA demethylating agents (Fernandez et al. 2009).
Different methylation pattern of L1 sequence has been found in carcinomas, premalignant lesions, and asymptomatic carriers in the case of HPV16 and HPV18 infection. HPV16 L1 sequence is methylated at intermediate level in asymptomatic infection, hypomethylated in precursor lesions, and hypermethylated in carcinomas (Badal et al. 2003; Kalantari et al. 2004). L1 gene of HPV-18 is also hypermethylated in the carcinomas contrasting with its hypomethylated state in asymptomatic infections and unmethylated in precursor lesions. These results suggest that L1 DNA methylation may be a powerful biomarker of the clinical progression of HPV-18-associated disease and possibly HPV-16-associated lesions as well (Turan et al. 2007).
Changes in DNA methylation pattern might also be found in the host genome. Several tumor suppressor genes possessing CpG islands in the promoter region are frequently inactivated by hypermethylation in cervical cancer cells (Szalmás and Kónya 2009; Woodman et al. 2007). Epigenetic silencing of genes involved in cell-cycle regulation (e.g., p16; Nakashima et al. 1999a, b; Nuovo et al. 1999), apoptosis (e.g., DcR1/DCR2, hTERT, p73; Shivapurkar et al. 2004; Widschwendter et al. 2004; Liu et al. 2004), DNA repair (MGMT; Narayan et al. 2003; Virmani et al. 2001), development and differentiation (RARβ; Narayan et al. 2003; Ivanova et al. 2002), hormonal response (ERα; Zambrano et al. 2005) and cellular signaling (RASSF1A; Cohen et al. 2003; Yu et al. 2003), invasion, and metastasis (DAPK; Narayan et al. 2003; Virmani et al. 2001) has been detected in cervical cancer cells. However, it is still not clear if methylation of tumor suppressor genes in cervical cancer cells is induced by HPV viruses or it is an effect of carcinogenesis. Difficulties with distinction may result from the fact that almost all of cervical cancer cells are HPV positive at diagnosis. Therefore, any comparison here will be non-informative opposite to (EBV)-positive and negative gastric cancers; hepatitis C positive and negative hepatocellular carcinomas, and simian virus 40 (SV 40) positive and negative mesotheliomas (Woodman et al. 2007).
Example of interactions between oncogenic viral proteins and host epigenetic machinery
Binds DNMT1 and stimulates DNA methyltransferase activity
Binds HDACs and Mi2 subunit of Nurd ATP-dependent remodeling complex
Induces KMD6A and KDM6B histone demethylase expression
Induces expression of histone methyltransferase EZH2
Interacts with p300/CBP and inhibits HAT activity
Activates DNMTs 1, 3a, and 3b
Interacts with p300 and activates transcription
Interacts with SUV39H1, MeCP2, and mSin3
Binds p300/CBP and inhibits HAT activity
Regulates the expression of DNMT3a and DNMT3b
Interacts with p300/CBP
Interacts with HDAC
Binds DNMT1 and stimulates DNA methyltransferase activity
Binds p300/CBP, TRRAP/GCN5, and PCAF HAT complexes
Binds to p400 and promotes the formation of a Myc–p400 complex at Myc-target gene promoters
Stimulates de novo H3K9me3 heterochromatin formation specifically at p53 target promoters
Interacts with p300/CBP to repress transcription
Binds BRG1 subunit of chromatin-remodeling complexes
The EBV is a human gamma-herpesvirus that predominantly establishes latent infection in B lymphocytes and epithelial cells. EBV is one of the most common viruses in humans. Ninety percent of the world’s population is infected by it (Young and Rickinson 2004; Williams and Crawford 2006; Klein et al. 2007). EBV is associated with mononucleosis and with several human cancers such as Burkitt’s lymphoma (BL; Bornkamm 2009), nasopharyngeal carcinoma (NPC), T- and NK-cell lymphoma, and gastric carcinoma (Fukayama et al. 2008). Moreover, EBV infection is involved in the etiology of several lymphoid and epithelial malignancies in immune-compromised humans, such as AIDS and posttransplant patients (Niller et al. 2008).
Double-stranded DNA genome of EBV viruses is huge, approximately 172 kb in size. In EBV infection, two stages can be distinguished, i.e., lytic and latent. During lytic life cycle, viruses are produced and finally released from the infected cells and viral genome remains as an episome in the host cell (Young and Rickinson 2004; Gatza et al. 2005; Williams and Crawford 2006; Klein et al. 2007). During latent infection, viral particles are not produced and several viral proteins called “latent proteins,” which have oncogenic activity, are expressed. The latency state is regulated by six EBV nuclear antigens EBNAs: 1, 2, 3A, 3B, 3C, and LP; three latent membrane proteins LMPs: 1, 2A, and 2B; BARF-1 protein; two small RNA molecules: EBER 1 and EBER2; and BART RNA transcripts. Additionally, EBV codes for at least 20 miRNAs that are expressed in latently infected cells (Tao et al. 1998; Klein et al. 2007; Bornkamm 2009). EBV genome also encodes for: immediate genes (probably responsible for the switch between latent and lytic cycle), the early genes (e.g., enzymes influencing the host cell nucleotide metabolism and DNA synthesis), and the late gene products (e.g., the virion structural proteins; Young and Rickinson 2004; Gatza et al. 2005; Williams and Crawford 2006; Klein et al. 2007).
The important role in carcinogenesis of all EBV positive tumors but Burkitt’s lymphoma, which is driven by the cMYC translocation, is played by LMP1 protein. LMP1 is one of the major EBV oncoprotein, which controls cell growth and promotes metastasis, apoptotic resistance, and immune modulation (Arvanitakis et al. 1995; Martin and Gutkind 2008). During B lymphocytes transformation LMP1 activates cell signaling pathways such as NFκB, inducing the expression of various genes that encode anti-apoptotic proteins and cytokines (Young and Rickinson 2004). LMP1 acts as a constitutively active receptor that mimics activated CD40, a member of the tumor necrosis factor family (Mosialos et al. 1995; Martin and Gutkind 2008). Critical role in EBV-induced transformation plays interaction between cytoplasmic carboxyl terminus of LMP1 and tumor necrosis factor receptor-associated factor and the tumor necrosis factor receptor-associated death domain protein (Brown et al. 2001). These interactions induce the activation of several key signaling molecules such as PI3K, JNK, and JAKs leading to the activation of transcription factors including NFκB, AP-1, and STATs (Kilger et al. 1998), which have been extensively related to human malignancies (Martin and Gutkind 2008).
In EBV-induced transformation as well as in EBV viral life cycle, epigenetic mechanisms such as DNA methylation and histone modifications, which control expression of latent viral oncogenes and miRNAs, play also an important role (Park et al. 2007b; Niller et al. 2008). Methylation of the EBV genome helps virus to hide from the host immune system, inhibiting expression of viral latency proteins that are recognized by cytotoxic T-cells (Robertson and Ambinder 1997; Paulson and Speck 1999; Tao and Robertson 2003; zur Hausen 2006; Fernandez et al. 2009). Methylation pattern of EBV genome depends on the stages of EBV latency (0, I, II, III) and the type of tumor (zur Hausen 2006). It has been demonstrated that certain viral promoters of latent circular EBV genomes may undergo increased methylation (Niller et al. 2008). DNA methylation level in EBV genome increases dramatically from asymptomatic infection to final neoplastic stages and has been shown to be involved in regulation of viral genes expression. One of the EBV genes whose expression is epigenetically regulated is EBNA1. EBNA1 protein plays crucial function in viral replication and episome maintenance in latency. Expression of EBNA 1 is controlled by four promoters; Cp, Wp, Qp, and Fp (Tao et al. 1998). DNA methylation of these promoters regulates the expression of EBNA1 and eventually defines the type of latency stage. CpG methylation downregulates gene expression and induces the alternative transcription of EBNA1 from various promoters during the different latency stages which, at the same time, are associated with the pathology that the virus induces from a simple infection to a lymphoma and carcinoma (Li and Minarovits 2003; Yoshioka et al. 2003; Niller et al. 2008). Wp, Cp, and X promoters have been found to be methylated in I and II latency type in BL, Hodgkin disease (HD), and NPC cells. Interestingly, Qp promoter remains unmethylated independently of its activity. It is suggested that it might be regulated by a putative repressor protein and specific histone modifications (Tao et al. 1998; Li and Minarovits 2003; Fejer et al. 2008; Fernandez et al. 2009).
Epigenetic mechanisms are also used by EBV virus to initiate lytic cycle and replication. This reactivation is initiated by the expression of the immediate-early BZLF1 gene, which encodes for the transcription activator Zta. This protein has the ability to bind to methylated sites and activate the expression of the remaining lytic genes, thereby inducing a lytic infection (Bhende et al. 2005; Countryman et al. 2008; Dickerson et al. 2009; Heather et al. 2009). DNA methylation also modulates expression of LMP2A in BL, HD, and NPC, but has no affect on the expression of major EBV oncogenic protein LMP1 (Young and Rickinson 2004). Analysis of CpG methylation pattern in EBV genome showed that only five promoters do not possess the DNA methylation mark: EBER1, EBER2, Qp, BZLF1, and LMP2B/LMP1 (Fernandez et al. 2009).
Expression of many tumor suppressor genes involved in the cell-cycle control, apoptosis, intracellular signaling, proliferation, and surface adhesion might be downregulated by DNA hypermethylation induced by EBV viral proteins. It has been demonstrated that LMP1 oncoprotein induces the activation of DNMT1 leading to an increase in methylation of tumor suppressor genes promoters in nasopharyngeal carcinoma cells (Tsai et al. 2002; Niemhom et al. 2008). Moreover, reduction of E-cadherin expression is the result of LMP1-induced hypermethylation by activation of DNA methyltransferases DNMT1 3A and 3B. (Tsai et al. 2002). Besides E-cadherin promoter, other tumor suppressor gene promoters such as RASSF1, retinoic acid receptor, β2, p16 INK4, and p14 are also hypermethylated in NPC cells (Lo et al. 2001; Lo et al. 2002; Kwong et al. 2002; Tong et al. 2002; Pai et al. 2007). Similarly, LMP2A protein intermediates in the activation of DNMT1 that leads to downregulation of PTEN gene expression in gastric carcinoma cells (Hino et al. 2009). Expression of host genes might also be affected by viral Zta protein which downregulates early growth response 1 which is a cellular transcription factor involved in diverse biological functions such as cell proliferation, apoptosis, and differentiation (Chang et al. 2006).
EBV oncoproteins might also interact with components of histone modification machinery. EBV viruses possess the ability to change histone modifications and chromatin structure. EBNA 2 and 3c alter histone acetylation by interaction with p300/CBP complex or with HDAC, respectively (Wang et al. 2000; Knight et al. 2003). Interestingly, all oncoproteins which interact with epigenetic regulators are latent genes which are not typically expressed in BL, gastric cancer, and most nasopharyngeal carcinomas (Flanagan 2007). Latest reports have demonstrated that LMP1, similar to HPV16 E7 oncoprotein, upregulate the expression of KDM6B demethylase (specific for H3K27me3) in Hodgkin’s lymphoma. It has been suggested that aberrant expression of KDM6B stimulated by LMP1 may contribute to the pathogenesis of HL. Moreover, the authors suggest that the changes in the distribution of the H3K27me3 mark, along with the dynamics of DNA methylation on early viral promoters, might also play a role in the latent/lytic switch (Anderton et al. 2011).
It has also been demonstrated that histone modifications play significant role in the activity of EBV promoters and expression of viral proteins (Gerle et al. 2007; Countryman et al. 2008; Fejer et al. 2008). Histone H3 lysine 4 dimethylation (H3K4me2) has been associated with Qp promoter activity and modulation of LMP2A expression. Acetylation of histone 3 and 4 has been linked with Qp, Cp promoter activity, and BZLF1 and LMP2A expression (Fernandez and Esteller 2010; Gerle et al. 2007; Countryman et al. 2008; Fejer et al. 2008).
EBV is the first virus that was reported to express miRNAs (Pfeffer et al. 2004). More than 20 miRNAs are encoded by EBV genome. They are differentially expressed in different phases of the viral life cycle and between the types of latency (Cai et al. 2006). EBV encoded miRNAs regulate both host and viral genes and have also been suggested to be implicated in the oncogenic properties of the virus (Pfeffer et al. 2004; Nair and Zavolan 2006; Gottwein and Cullen 2008; Takacs et al. 2010; Moens 2009).
Kaposi’s sarcoma-associated herpesvirus
KSHV also known as human herpesvirus 8 likewise EBV belongs to the Herpesviridae family. KSHV is associated with Kaposi sarcoma, one of the most common cancer in human immune deficiency virus-infected patients, primary effusion lymphoma (PEL), and some type of multicentric Castleman’s disease (zur Hausen 2006). Similar to EBV virus, KSHV is a dsDNA virus, whose infection persists for life and it posseses two phase in its life cycle, i.e., latent and lytic. Seven KSHV genes are closely associated with latency and have potentially oncogenic activity: LANA, vcyclinD, vFLIP (K13), Kaposin (K12), vIRF2 (K11.5), vIRF3 (K10.5), and LAMP (K15) (Areste’ and Blackbourn 2009; Dourmishev et al. 2003; zur Hausen 2006) All of these proteins have the ability to maintain lytic phase and to control latent cycle replication. Replication and transcription activator (RTA) is encoded by ORF50 of the viral genome and is the lytic switch of KSHV (Sun et al. 1998). Methylation of RTA (ORF50) promoter is used by virus to maintain the latent cycle. The main latency protein LANA (latency-associated nuclear protein) which remains unmethylated during KSHV infection also supports maintenance of the latent cycle by the association with ORF50 promoter or binding cellular factors which normally interact with ORF50 (Lu et al. 2006; Pantry and Medveczky 2009).
It has been shown that KSHV may influence host DNA methylation. LANA protein has been demonstrated to associate with DNA methyltransferase DNMT3a, which results in repression of approximately 80 cellular genes, some of which are known targets of epigenetic inactivation in various cancers (Shamay et al. 2006). Association and relocalization of DNMT3a induced by LANA has an influence on methylation of the H-cadherin gene promoter. It has also been reported that LANA associates with the TGF-β type II receptor (TβRII) promoter and induces its methylation (Di Bartolo et al. 2008). Reduction of TβRII expression in PEL cells results in defective TGF-β signaling-pathway, which is important for preventing the development of tumors because it inhibits growth and promotes apoptosis (Di Bartolo et al. 2008). Another tumor suppressor, p16INK4a, is also found to be inactivated by promoter hypermethylation. However, it has not been proved that LANA participates in its downregulation.
KSHV oncoproteins also interact with other components of epigenetic machinery. LANA protein interacts with the DNA methyl-binding protein MeCP2, the mSin3 transcriptional repression complex, and the histone methyltransferase SUV39H1, thus enabling numerous roles in epigenetic gene regulation (Flanagan 2007; Li et al. 2000). LANA, RTA, K-bZip, and viral homologue of interferon regulatory factor encoded by ORFK9 interact with histone acetyltransferase complex p300/CBP and lead to reduction of its activity (Li et al. 2000; Hwang et al. 2001; Lim et al. 2001; Gwack et al. 2001, 2002; Pantry and Medveczky 2009). Moreover, miRNAs encoded by KSHV virus are also involved in epigenetic regulation and expression of oncogenes (Cai et al. 2006; Flanagan 2007; Samols et al. 2007).
Hepatitis B virus
HBV is a member of Hepadnaviridae family. Viruses that belong to this family cause acute and chronic infections of the liver resulting in cirrhosis, hepatitis B, and HCC (Beck and Nassal 2007; Seeger and Mason 2000; zur Hausen 2006). HBV and HCV are the main factors responsible for HCC development in humans worldwide (Cougot et al. 2005; Gurtsevitch 2008). HBV contains a double-stranded circular DNA genome of 3.2 kb and it replicates by reverse transcription from an RNA intermediate (pregenomic RNA), which is transcribed from covalently closed circular HBV DNA (Yokosuka and Arai 2006).
In contrast to HCV infection during HBV replication, epigenetic mechanisms such as DNA methylation or histone modifications play an important role. Almost completely unmethylated HBV genome occurs in the early stages of carcinogenesis (e.g., hepatitis and cirrhosis); whereas, HBV genome is more methylated in the established liver tumors, both in clinical samples as well as in cultured cancer cell lines (Fernandez et al. 2009). The presence of DNA methylation at the C and S genes is related to their lack of expression. Conversely, X gene that encodes for HBx protein remains unmethylated (Fernandez et al. 2009). HBx oncoprotein, which plays an important role in carcinogenesis, is also a key factor responsible for epigenetic alteration in viral and host genome (Jung et al. 2007; Park et al. 2007a; Zheng et al. 2009). HBx protein interacts with DNMT1 and has influence on its expression. Increased expression of DNMT1 induced by HBx inhibits the expression of tumor suppressor genes such as p16 and E-cadherin (Jung et al. 2007). Moreover, HBx directly interacts and regulates the expression of DNMT3a and DNMT3b which also modulates host genes expression (Park et al. 2007a; Zheng et al. 2009). The same mechanisms are used by HBx to control viral genome methylation pattern (Jung et al. 2007; Park et al. 2007a; Zheng et al. 2009). HBx has also been demonstrated to associate with components of histone modification machinery, such as CBP/p300 HAT and HDAC, thus influencing gene expression (Cougot et al. 2007; Shon et al. 2009; Zheng et al. 2009).
Human Adenoviruses are small DNA viruses with non-enveloped icohasedral capsid (Russell 2009). More than 50 human serotypes of adenoviruses have been identified and subdivided into groups A to F (Blackford and Grand 2009; Russell 2009). Adenoviruses mostly cause respiratory infections but a subset of them containing all subgroup A and B (e.g., Ad12) are capable of promoting undifferentiated tumors when injected into rodents (Graham et al. 1984; Täuber and Dobner 2001; for a comprehensive review, see Doerfler 2009). Nevertheless, role of adenoviruses in human carcinogenesis is still unclear. It has been suggested that adenoviruses might not cause human cancers due to the fact that adenoviral DNA was generally not detected in human tumor cells. However, latest data indicate that adenoviruses can establish a form of latency in some human cells (Garnett et al. 2009). Moreover, because adenoviral DNA has been detected in brain tumors (Kosulin et al. 2007) then potential involvement of adenoviruses in human carcinogenesis should be considered. It has been proposed that adenoviruses might perform “hit and run” transformation of human cells (Nevels et al. 2001). According to this hypothesis, cellular transformation may be caused by transient viral infection, and after establishing neoplastic state of the cell, viral DNA is not necessary for the maintenance of transformed cellular phenotype. Studies on Syrian hamster cells transformed by Ad12 have demonstrated that despite of the gradual loss of multiple copies of integrated Ad12 genomes from these cells, their oncogenic potential was still maintained (Doerfler 2009). The “hit and run” oncogenesis concept could explain the role of adenoviruses as etiological agents in tumors that lack any viral genes and proteins (Nevels et al. 2001).
Oncogenic properties of adenoviruses have been attributed mainly to the function of early region 1 (E1) which encodes Ad E1A and E1B oncoproteins (Täuber and Dobner 2001). Apart from E1A and E1B oncoproteins, proteins encoded by E4 region have also been proposed to be involved in cellular transformation. Early proteins E4-ORF3 and E4-ORF6 of adenovirus Ad5 have been shown to be able of replacing E1B function in transforming cells and to increase cellular transformation mediated by E1A and E1B as well (Täuber and Dobner 2001). Early viral proteins E1A and E1B act as transcriptional factors involved in the regulation of viral and cellular gene expression and have been demonstrated to interact with many cellular proteins including tumor suppressors, RB proteins, and p53, respectively (Endter and Dobner 2004; Martin and Berk 1998; Kosulin et al. 2007; Zheng 2010).
Epigenetic alterations such as changes in DNA methylation pattern of viral and host genome as well as histone modification have been extensively demonstrated to occur in adenovirus-transformed cells. It has been observed that integrated adenoviral (Ad12) genome in hamster tumor cells becomes de novo methylated, contrary to free viral DNA, which is never methylated de novo (Doerfler 1991a, b; 1996; 2009). Moreover, changes in methylation pattern of cellular genome were found in cells which have Ad12 DNA integrated into host genome. However, it is unknown whether changes in methylation profile of cellular genomes are induced by insertion of adenoviral DNA or just by insertion of any foreign DNA (Doerfler 2009).
Many studies have also demonstrated that adenoviral oncoproteins interact with components of cellular epigenetic machinery. These interactions are another example of functional convergence of oncoproteins encoded by adenoviruses and HPV viruses. For instance, E1A correspondingly to E7HPV16 protein associates with the DNMT1 and increases its activity (Burgers et al. 2007). Moreover, E1A similar to E6 HPV16 protein binds to and inhibits HAT proteins CBP and p300. It has been shown that interaction between E1A and CBP/p300 leads to reduction of histone H3 lysine 18 acetylation (H3K18ac; Horwitz et al. 2008; Ferrari et al. 2009). In addition, E1A also binds to several other cellular proteins such as GCN5, PCAF, and p400, which are involved in the regulation of chromatin structure (Lang and Hearing 2003; Fuchs et al. 2001; Horwitz et al. 2008; Ferrari et al. 2009). Recent studies have demonstrated that E1A function results in epigenetic reprogramming of the host cell. It has been shown that E1A binds in a time-dependent manner to promoter regions of diverse sets of biologically related cellular genes which causes genome-wide redistribution of RB proteins and CBP/p300 on promoters, hypoacetylation of H3K18 in these regions, and subsequent target genes repression, which results in transcriptional reprogramming of the cell (Ferrari et al. 2008). Furthermore, recent studies have demonstrated that function of adenoviral E4-ORF3 protein may also induce extensive epigenetic alteration in transformed cells. It has been shown that E4-ORF3 stimulates de novo H3K9me3 heterochromatin formation specifically at p53 target promoters, thus leading to an inhibition of p53 DNA binding and silencing of p53-target genes transcription (Soria et al. 2010).
HTLV-1 is a complex retrovirus with a single-stranded RNA genome that is associated with multiple diseases including an aggressive clonal malignancy of mature CD4+ T-lymphocytes called adult T-cell leukemia/lymphoma (ATL). It is also responsible for causing chronic inflammatory disease called HAM/TSP for HTLV-1-associated myelopathy/tropical spastic paraparesis (Araujo and Silva 2006). At present, HTLV-1 is still the only known human retrovirus directly linked to oncogenesis. It is estimated that about 20 million people worldwide are infected with HTLV-1 (Proietti et al. 2005). In spite of this, ATL develops only in minority of HTLV-1-infected individuals. The risk of ATL in HTLV-1-infected people is estimated to be approximately 6.6% for males and 2.1% for females (Arisawa et al. 2000). The causative role of HTLV-1 in ATL etiology is well documented. ATL develops only in HTLV-1 carriers. Moreover, it has been shown that all ATL cells contain integrated HTLV-1 provirus.
In contrast to mechanisms typical for animal retroviruses, HTLV-1-mediated oncogenesis involves virally encoded proteins rather than insertional mutagenesis or capturing and activating cellular proto-oncogenes (Yoshida 2001, 2005; Matsuoka and Jeang 2007). The main transforming protein of HTLV-1 is Tax oncoprotein, but recent studies evidence that the basic leucine zipper factor (HBZ) also plays a role in this process (Matsuoka and Jeang 2007). It has been proposed that Tax protein is needed to initiate ATL transformation, and HBZ protein is involved in leukemia maintenance (Matsuoka and Jeang 2007). Although Tax protein is required for the virus to transform T-cells, its transcripts are detected in only about 40% of all ATLs. It has been demonstrated that Tax expression is silenced in ATL cells, which enables transformed cells to evade immunosurveillance (Koiwa et al. 2002; Takeda et al. 2004; Taniguchi et al. 2005; Matsuoka and Jeang 2007).
Tax protein contributes to the initiation of T-cell transformation through various mechanisms, by deregulating the function and expression of key cellular factors involved in cell growth and proliferation, apoptosis, DNA repair, and cell division (Matsuoka and Jeang 2007). It has been demonstrated that Tax protein associates with centrosomes, causing their amplification and as a consequence multipolar mitosis and aneuploidy. It abrogates DNA repair which contributes to clastogenic DNA damage in HTLV-1-infected cells. It is also responsible for inactivation of factors involved in DNA damage response, e.g., p53, thus contributing to suppression of apoptosis and senescence. Other reported mechanisms of Tax-mediated transformation include activation of cyclin-dependant kinases, NFκB, and Akt signaling which promote cell survival and proliferation of HTLV-1 infected cells.
Many studies report that Tax protein also influences host cell epigenetic machinery. It has been shown that Tax protein forms complex with the phosphorylated form of the cellular transcription factor pCREB that recruits the cellular histone acetyltransferases CBP/p300 to promote changes in chromatin architecture characteristic for transcriptional activation. This mechanism of Tax-mediated change in histone acetylation is used by HTLV-1 to activate transcription of viral genes from viral long terminal repeats LTR and is required for high-level transcription of the proviral DNA. Recent evidences also show that Tax interacts with BRG1 subunit of chromatin-remodeling complexes. This interaction leads to HTLV-1 nucleosome remodeling and is required for Tax transactivation.
It has also been demonstrated that Tax protein can recruit histone methyltransferase SUV39H1 to 5′LTR and induce H3K9 methylation whereby it modulates its own expression which plays a role in the regulation of viral latency (Kamoi et al. 2006).
The studies in characterizing the molecular mechanisms of viral-induced carcinogenesis provide increasing evidence for the importance of the interactions between viruses and host cells at the epigenetic level. It is now apparent that viral oncoproteins target the elements of cellular epigenetic machinery changing their expression and/or activity thus leading to alterations in the epigenetic state of the host cell. Viral-encoded oncoproteins exploit specific epigenetic processes to force normal quiescent cells to replicate as well as to regulate viral genes expression during infections. DNA methylation in viral promoters modulates viral genes expression and is the mechanism used by many oncoviruses to avoid detection by the host immune system.
Epigenetic alterations in DNA methylation and histone modifications, leading to aberrant profiles of gene expression, are highly conserved function in tumor-associated viruses belonging to distinct evolutionary groups. Common targets for the viral oncoproteins are DNA methyltransferases (maintenance DNA methyltransferase, DNMT1, and/or de novo DNA methyltransferases, DNMT3a, DNMT3b) and histone-modifying enzymes, such as HDAC, HAT, histone methyltransferases, and demethylases. Emerging data also point toward a role of miRNA in the regulation of viral life cycle and pathogenesis of several virus-associated cancers. However, while changes in DNA methylation pattern and some histone modification changes induced by viral infection are better recognized, the function of miRNA still remains poorly understood.
The fact that oncovirus-induced epigenetic alterations within host cell during carcinogenesis are also a characteristic for most non-viral cancers demonstrates the similarity between the viral and tumor cell programs at the epigenetic level. Aberrant methylation patterns are an important and frequent event both in virus-associated and non-viral cancers (Robertson 2001; Jones and Baylin 2002). Many studies demonstrate an essential role of elevated Dnmt1, Dnmt3a, and Dnmt3b expression and activity in the development of cancers (Robertson 2001; Esteller 2006). The resulting hypermethylation of CpG island promoter observed in many cancers has been widely demonstrated to cause silencing of tumor suppressor genes. Aberrant histone modifications in particular histone acetylation, which lead to misregulation in gene expression, are also a characteristic feature of human cancer cells. Inhibition of p300/CBP histone acetyltransferase activity is observed in many non-viral cancers. The germline mutations of CBP are found in Rubinstein–Taybi syndrome, a developmental disorder characterized by an increased predisposition to childhood malignancies, e.g., solid tumors, leukemias, and lymphomas. Moreover, frequent somatic mutations of p300/CBP have been detected in breast, colorectal, and gastric carcinomas (Iyer et al. 2004). Altered expression and mutations of genes that encode HDACs have also been associated with carcinogenesis (Ropero and Esteller 2007 and references therein). Overexpression of individual HDACs has been detected in many different tumors, but there are also evidences that alterations that result in the loss of function of class I HDACs may also be associated with cancer development. It has been proposed that the loss of class I HDAC function could induce the hyperacetylation and activation of genes regulated by RB protein, thus leading to cell-cycle deregulation (Ropero and Esteller 2007). Aberrant histone methylation has also been widely demonstrated to contribute to carcinogenesis. Deregulation of H3K27 methylation caused by both increased and decreased activity of enzymes controlling H3K27 methylation is observed in many cancers, which demonstrates that precise balance of this methylation plays an important role in normal cell growth (Simon and Lange 2008; Martinez-Garcia and Licht 2010).
In light of the discussed significance of epigenetic mechanisms in tumorigenesis, oncogenic viruses can be seen as important players changing the function of cellular epigenetic machinery, thereby contributing to cancer development. The models of virus-induced epigenetic reprogramming may also apply to non-viral mechanisms of oncogenesis. Therefore, the results of studies aimed at complete understanding of the viral interference with the cellular epigenetic processes will have a powerful impact also on understanding of the epigenetic mechanisms involved in human non-viral carcinogenesis.
Work in Department of Molecular Virology is supported by the Polish State Committee for Scientific Research grant nos. NN303813140, NN401012136, and NN401219824.
Conflict of interest
The authors declare no conflict of interest.
- Anderton JA, Bose S, Vockerodt M, Vrzalikova K, Wei W, Kuo M, Helin K, Christensen J, Rowe M, Murray PG, Woodman CB (2011) The H3K27me3 demethylase, KDM6B, is induced by Epstein–Barr virus and over-expressed in Hodgkin’s lymphoma. Oncogene. doi:https://doi.org/10.1038/onc.2010.579
- Araujo AQ, Silva MT (2006) The HTLV-1 neurological complex. Lancet Neurol 5(12):1068–1076. doi:https://doi.org/10.1016/S1474-4422(06)70628-7PubMedGoogle Scholar
- Areste’ C, Blackbourn DJ (2009) Modulation of the immune system by Kaposi’s sarcoma-associated herpesvirus. Trends Microbiol 17:119–129. doi:https://doi.org/10.1016/j.tim.2008.12.001Google Scholar
- Arisawa K, Soda M, Endo S, Kurokawa K, Katamine S, Shimokawa I, Koba T, Takahashi T, Saito H, Doi H, Shirahama S (2000) Evaluation of adult T-cell leukemia/lymphoma incidence and its impact on non-Hodgkin lymphoma incidence in southwestern Japan. Int J Cancer 85(3):319–324. doi:https://doi.org/10.1002/(SICI)1097-0215(20000201)85%3A3<319%3A%3AAID-IJC4>3.0.CO%3B2-BPubMedGoogle Scholar
- Arvanitakis L, Yaseen N, Sharma S (1995) Latent membrane protein-1 induces cyclin D2 expression, pRb hyperphosphorylation, and loss of TGF-beta 1-mediated growth inhibition in EBV-positive B cells. J Immunol 155(3):1047–1056PubMedGoogle Scholar
- Avvakumov N, Torchia J, Mymryk JS (2003) Interaction of the HPV E7 proteins with the pCAF acetyltransferase. Oncogene 22(25):3833–3841. doi:https://doi.org/10.1038/sj.onc.1206562PubMedGoogle Scholar
- Badal V, Chuang LS, Tan EH, Badal S, Villa LL, Wheeler CM, Li BF, Bernard HU (2003) CpG methylation of human papillomavirus type 16 DNA in cervical cancer cell lines and in clinical specimens: genomic hypomethylation correlates with carcinogenic progression. J Virol 77(11):6227–6234. doi:https://doi.org/10.1128/JVI.77.11.6227-6234.2003PubMedPubMed CentralGoogle Scholar
- Badal S, Badal V, Calleja-Macias IE, Kalantari M, Chuang LS, Li BF, Bernard HU (2004) The human papillomavirus-18 genome is efficiently targeted by cellular DNA methylation. Virology 324(2):483–492. doi:https://doi.org/10.1016/j.virol.2004.04.002PubMedGoogle Scholar
- Beck J, Nassal M (2007) Hepatitis B virus replication. World J Gastroenterol 13:48–64PubMedPubMed CentralGoogle Scholar
- Bhattacharjee B, Sengupta S (2006) CpG methylation of HPV 16 LCR at E2 binding site proximal to P97 is associated with cervical cancer in presence of intact E2. Virology 354(2):280–285. doi:https://doi.org/10.1016/j.virol.2006.06.018PubMedGoogle Scholar
- Bhende PM, Seaman WT, Delecluse HJ, Kenney SC (2005) BZLF1 activation of the methylated form of the BRLF1 immediate-early promoter is regulated by BZLF1 residue 186. J Virol 79:7338–7348. doi:https://doi.org/10.1128/JVI.79.12.7338-7348.2005PubMedPubMed CentralGoogle Scholar
- Biancotto C, Frigè G, Minucci S (2010) Histone modification therapy of cancer. Adv Genet 70:341–386. doi:https://doi.org/10.1016/B978-0-12-380866-0.60013-7PubMedGoogle Scholar
- Blackford AN, Grand RJ (2009) Adenovirus E1B 55-kilodalton protein: multiple roles in viral infection and cell transformation. J Virol 83(9):4000–4012. doi:https://doi.org/10.1128/JVI.02417-08PubMedPubMed CentralGoogle Scholar
- Bornkamm GW (2009) Epstein–Barr virus and the pathogenesis of Burkitt’s lymphoma: more questions than answers. Int J Cancer 124:1745–1755. doi:https://doi.org/10.1002/ijc.24223PubMedGoogle Scholar
- Brandsma JL, Sun Y, Lizardi PM, Tuck DP, Zelterman D, Haines GK 3rd, Martel M, Harigopal M, Schofield K, Neapolitano M (2009) Distinct human papillomavirus type 16 methylomes in cervical cells at different stages of premalignancy. Virology 389(1–2):100–107. doi:https://doi.org/10.1016/j.virol.2009.03.029PubMedPubMed CentralGoogle Scholar
- Brown KD, Hostager BS, Bishop GA (2001) Differential signaling and tumor necrosis factor receptor-associated factor (TRAF) degradation mediated by CD40 and the Epstein–Barr virus oncoprotein latent membrane protein 1 (LMP1). J Exp Med 193(8):943–954. doi:https://doi.org/10.1084/jem.193.8.943PubMedPubMed CentralGoogle Scholar
- Burgers WA, Blanchon L, Pradhan S, de Launoit Y, Kouzarides T, Fuks F (2006) Viral oncoproteins target the DNA methyltransferases. Oncogene 26(11):1650–1655. doi:https://doi.org/10.1038/sj.onc.1209950PubMedPubMed CentralGoogle Scholar
- Burgers WA, Blanchon L, Pradhan S, de Launoit Y, Kouzarides T, Fuks F (2007) Viral oncoproteins target the DNA methyltransferases. Oncogene 26(11):1650. doi:https://doi.org/10.1038/sj.onc.1209950PubMedPubMed CentralGoogle Scholar
- Butel JS (2000) Viral carcinogenesis: revelation of molecular mechanisms and etiology of human disease. Carcinogenesis 21:405–426. doi:https://doi.org/10.1093/carcin/21.3.405PubMedGoogle Scholar
- Cai X, Schäfer A, Lu S, Bilello JP, Desrosiers RC, Edwards R, Raab-Traub N, Cullen BR (2006) Epstein–Barr virus microRNAs are evolutionarily conserved and differentially expressed. PLoS Pathog 2:e23. doi:https://doi.org/10.1371/journal.ppat.0020023PubMedPubMed CentralGoogle Scholar
- Chang Y, Lee HH, Chen YT, Lu J, Wu SY, Chen CW, Takada K, Tsai CH (2006) Induction of the early growth response 1 gene by Epstein–Barr virus lytic transactivator Zta. J Virol 80:7748–7755. doi:https://doi.org/10.1128/JVI.02608-05PubMedPubMed CentralGoogle Scholar
- Chi P, Allis CD, Wang GG (2010) Covalent histone modifications—miswritten, misinterpreted and mis-erased in human cancers. Nat Rev Cancer 10(7):457–469. doi:https://doi.org/10.1038/nrc2876PubMedPubMed CentralGoogle Scholar
- Ciuffo G (1907) Innesto positivo con filtrato di verruca volgare. Giorn Ital Mal Venereol 48:12–17Google Scholar
- Cohen Y, Singer G, Lavie O, Dong SM, Beller U, Sidransky D (2003) The RASSF1A tumor suppressor gene is commonly inactivated in adenocarcinoma of the uterine cervix. Clin Cancer Res 9(8):2981–2984PubMedGoogle Scholar
- Cougot D, Neuveut C, Buendia MA (2005) HBV induced carcinogenesis. J Clin Virol 34:S75–S78. doi:https://doi.org/10.1016/S1386-6532(05)80014-9PubMedGoogle Scholar
- Cougot D, Wu Y, Cairo S, Caramel J, Renard CA, Lévy L, Buendia MA, Neuveut C (2007) The hepatitis B virus X protein functionally interacts with CREB-binding protein/p300 in the regulation of CREB-mediated transcription. J Biol Chem 282:4277–4287. doi:https://doi.org/10.1074/jbc.M606774200PubMedGoogle Scholar
- Countryman JK, Gradoville L, Miller G (2008) Histone hyperacetylation occurs on promoters of lytic cycle regulatory genes in Epstein–Barr virus-infected cell lines which are refractory to disruption of latency by histone deacetylase inhibitors. J Virol 82:4706–4719. doi:https://doi.org/10.1128/JVI.00116-08PubMedPubMed CentralGoogle Scholar
- de Villiers EM, Fauquet C, Broker TR, Bernard HU, zur Hausen H (2004) Classification of papillomaviruses. Virology 324(1):17–27. doi:https://doi.org/10.1016/j.virol.2004.03.033PubMedGoogle Scholar
- Di Bartolo DL, Cannon M, Liu YF, Renne R, Chadburn A, Boshoff C, Cesarman E (2008) KSHV LANA inhibits TGF-beta signaling through epigenetic silencing of the TGF-beta type II receptor. Blood 111:4731–4740. doi:https://doi.org/10.1182/blood-2007-09-110544PubMedPubMed CentralGoogle Scholar
- Dickerson SJ, Xing Y, Robinson AR, Seaman WT, Gruffat H, Kenney SC (2009) Methylation-dependent binding of the Epstein–Barr virus BZLF1 protein to viral promoters. PLoS Pathog 5:e1000356. doi:https://doi.org/10.1371/journal.ppat.1000356PubMedPubMed CentralGoogle Scholar
- Doerfler W (1991a) Abortive infection and malignant transformation by adenoviruses: integration of viral DNA and control of viral gene expression by specific patterns of DNA methylation. Adv Virus Res 39:89–128PubMedGoogle Scholar
- Doerfler W (1991b) Patterns of DNA methylation—evolutionary vestiges of foreign DNA inactivation as a host defense mechanism. A proposal. Biol Chem Hoppe Seyler 372:557–564PubMedGoogle Scholar
- Doerfler W (1996) A new concept in (adenoviral) oncogenesis: integration of foreign DNA and its consequences. Biochim Biophys Acta 1288(2):F79–F99. doi:https://doi.org/10.1016/0304-419X(96)00024-8PubMedGoogle Scholar
- Doerfler W (2009) Epigenetic mechanisms in human adenovirus type 12 oncogenesis. Semin Cancer Biol 19(3):136–143. doi:https://doi.org/10.1016/j.semcancer.2009.02.009PubMedGoogle Scholar
- Doorbar J (2005) The papillomavirus life cycle. J Clin Virol 32(Suppl 1):S7–S15. doi:https://doi.org/10.1016/j.jcv.2004.12.006PubMedGoogle Scholar
- Doorbar J (2006) Molecular biology of human papillomavirus infection and cervical cancer. Clin Sci Lond 110(5):525–541. doi:https://doi.org/10.1042/CS20050369PubMedGoogle Scholar
- Dourmishev LA, Dourmishev AL, Palmeri D, Schwartz RA, Lukac DM (2003) Molecular genetics of Kaposi’s sarcoma-associated herpesvirus (human herpesvirus-8) epidemiology and pathogenesis. Microbiol Mol Biol Rev 67:175–212. doi:https://doi.org/10.1128/MMBR.67.2.175-212.2003PubMedPubMed CentralGoogle Scholar
- Endter C, Dobner T (2004) Cell transformation by human adenoviruses. Curr Top Microbiol Immunol 273:163–214PubMedGoogle Scholar
- Esteller M (2006) Epigenetics provides a new generation of oncogenes and tumour-suppressor genes. Br J Cancer 94(2):179–183. doi:https://doi.org/10.1038/sj.bjc.6602918PubMedPubMed CentralGoogle Scholar
- Esteller M (2007) Cancer epigenomics: DNA methylomes and histone-modification maps. Nat Rev Genet 8(4):286–298. doi:https://doi.org/10.1038/nrg2005PubMedGoogle Scholar
- Fejer G, Koroknai A, Banati F, Györy I, Salamon D, Wolf H, Niller HH, Minarovits J (2008) Latency type-specific distribution of epigenetic marks at the alternative promoters Cp and Qp of Epstein–Barr virus. J Gen Virol 89:1364–1370. doi:https://doi.org/10.1099/vir.0.83594-0PubMedGoogle Scholar
- Feng H, Shuda M, Chang Y, Moore PS (2008) Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science 319:1096–1100. doi:https://doi.org/10.1126/science.1152586PubMedPubMed CentralGoogle Scholar
- Fernandez AF, Esteller M (2010) Viral epigenomes in human tumorigenesis. Oncogene 29(10):1405–1420. doi:https://doi.org/10.1038/onc.2009.517PubMedGoogle Scholar
- Fernandez AF, Rosales C, Lopez-Nieva P, Graña O, Ballestar E, Ropero S, Espada J, Melo SA, Lujambio A, Fraga MF, Pino I, Javierre B, Carmona FJ, Acquadro F, Steenbergen RD, Snijders PJ, Meijer CJ, Pineau P, Dejean A, Lloveras B, Capella G, Quer J, Buti M, Esteban JI, Allende H, Rodriguez-Frias F, Castellsague X, Minarovits J, Ponce J, Capello D, Gaidano G, Cigudosa JC, Gomez-Lopez G, Pisano DG, Valencia A, Piris MA, Bosch FX, Cahir-McFarland E, Kieff E, Esteller M (2009) The dynamic DNA methylomes of double-stranded DNA viruses associated with human cancer. Genome Res 19(3):438–451. doi:https://doi.org/10.1101/gr.083550.108PubMedPubMed CentralGoogle Scholar
- Ferrari R, Pellegrini M, Horwitz GA, Xie W, Berk AJ, Kurdistani SK (2008) Epigenetic reprogramming by adenovirus e1a. Science 321(5892):1086–1088. doi:https://doi.org/10.1126/science.1155546PubMedPubMed CentralGoogle Scholar
- Ferrari R, Berk AJ, Kurdistani SK (2009) Viral manipulation of the host epigenome for oncogenic transformation. Nat Rev Genet 10:290–294. doi:https://doi.org/10.1038/nrg2539PubMedPubMed CentralGoogle Scholar
- Flanagan JM (2007) Host epigenetic modifications by oncogenic viruses. Br J Cancer 96:183–188. doi:https://doi.org/10.1038/sj.bjc.6603516PubMedPubMed CentralGoogle Scholar
- Fuchs M, Gerber J, Drapkin R, Sif S, Ikura T, Ogryzko V, Lane WS, Nakatani Y, Livingston DM (2001) The p400 complex is an essential E1A transformation target. Cell 106(3):297–307. doi:https://doi.org/10.1016/S0092-8674(01)00450-0PubMedGoogle Scholar
- Fukayama M, Hino R, Uozaki H (2008) Epstein–Barr virus and gastric carcinoma: virus–host interactions leading to carcinoma. Cancer Sci 99:1726–1733. doi:https://doi.org/10.1111/j.1349-7006.2008.00888.xPubMedGoogle Scholar
- Garnett CT, Talekar G, Mahr JA, Huang W, Zhang Y, Ornelles DA, Gooding LR (2009) Latent species C adenoviruses in human tonsil tissues. J Virol 83(6):2417–2428. doi:https://doi.org/10.1128/JVI.02392-08PubMedPubMed CentralGoogle Scholar
- Gatza ML, Chandhasin C, Ducu RI, Marriott SJ (2005) Impact of transforming viruses on cellular mutagenesis, genome stability, and cellular transformation. Environ Mol Mutagen 45(2–3):304–325. doi:https://doi.org/10.1002/em.20088PubMedGoogle Scholar
- Gerle B, Koroknai A, Fejer G, Bakos A, Banati F, Szenthe K, Wolf H, Niller HH, Minarovits J, Salamon D (2007) Acetylated histone H3 and H4 mark the upregulated LMP2A promoter of Epstein–Barr virus in lymphoid cells. J Virol 81(23):13242–13247. doi:https://doi.org/10.1128/JVI.01396-07PubMedPubMed CentralGoogle Scholar
- Goel A, Li M-S, Nagasaka T, Shin SK, Fuerst F, Ricciardiello L et al (2006) Association of JC virus T-antigen expression with the methylator phenotype in sporadic colorectal cancers. Gastroenterology 130:1950–1961. doi:https://doi.org/10.1053/j.gastro.2006.02.061PubMedGoogle Scholar
- Gottwein E, Cullen BR (2008) Viral and cellular MicroRNAs as determinants of viral pathogenesis and immunity. Cell Host Microbe 3:375–387. doi:https://doi.org/10.1016/j.chom.2008.05.002PubMedPubMed CentralGoogle Scholar
- Graham FL, Rowe DT, McKinnon R, Bacchetti S, Ruben M, Branton PE (1984) Transformation by human adenoviruses. J Cell Physiol Suppl 3:151–163PubMedGoogle Scholar
- Gurtsevitch VE (2008) Human oncogenic viruses: hepatitis B and hepatitis C viruses and their role in hepatocarcinogenesis. Biochem Mosc 73:504–513. doi:https://doi.org/10.1134/S0006297908050039Google Scholar
- Gwack Y, Hwang S, Byun H, Lim C, Kim JW, Choi EJ, Choe J (2001) Kaposi’s sarcoma-associated herpesvirus open reading frame 50 represses p53-induced transcriptional activity and apoptosis. J Virol 75(13):6245–6248. doi:https://doi.org/10.1128/JVI.75.13.6245-6248.2001PubMedPubMed CentralGoogle Scholar
- Gwack Y, Hwang S, Lim C, Won YS, Lee CH, Choe J (2002) Kaposi’s sarcoma-associated herpesvirus open reading frame 50 stimulates the transcriptional activity of STAT3. J Biol Chem 277(8):6438–6442. doi:https://doi.org/10.1074/jbc.M108289200PubMedGoogle Scholar
- Heather J, Flower K, Isaac S, Sinclair AJ (2009) The Epstein–Barr virus lytic cycle activator Zta interacts with methylated ZRE in the promoter of host target gene egr1. J Gen Virol 90:1450–1454. doi:https://doi.org/10.1099/vir.0.007922-0PubMedPubMed CentralGoogle Scholar
- Hino R, Uozaki H, Murakami N, Ushiku T, Shinozaki A, Ishikawa S, Morikawa T, Nakaya T, Sakatani T, Takada K, Fukayama M (2009) Activation of DNA methyltransferase 1 by EBV latent membrane protein 2A leads to promoter hypermethylation of PTEN gene in gastric carcinoma. Cancer Res 69:2766–2774. doi:https://doi.org/10.1158/0008-5472.CAN-08-3070PubMedGoogle Scholar
- Holland D, Hoppe-Seyler K, Schuller B, Lohrey C, Maroldt J, Dürst M, Hoppe-Seyler F (2008) Activation of the enhancer of zeste homologue 2 gene by the human papillomavirus E7 oncoprotein. Cancer Res 68(23):9964–9972. doi:https://doi.org/10.1158/0008-5472.CAN-08-1134PubMedGoogle Scholar
- Horwitz GA, Zhang K, McBrian MA, Grunstein M, Kurdistani SK, Berk AJ (2008) Adenovirus small e1a alters global patterns of histone modification. Science 321(5892):1084–1085. doi:https://doi.org/10.1126/science.1155544PubMedPubMed CentralGoogle Scholar
- Hublarova P, Hrstka R, Rotterova P, Rotter L, Coupkova M, Badal V, Nenutil R, Vojtesek B (2009) Prediction of human papillomavirus 16 e6 gene expression and cervical intraepithelial neoplasia progression by methylation status. Int J Gynecol Cancer 19:321–325PubMedGoogle Scholar
- Hwang S, Gwack Y, Byun H, Lim C, Choe J (2001) The Kaposi’s sarcoma-associated herpesvirus K8 protein interacts with CREB-binding protein (CBP) and represses CBP-mediated transcription. J Virol 75(19):9509–9516. doi:https://doi.org/10.1128/JVI.75.19.9509-9516.2001PubMedPubMed CentralGoogle Scholar
- Ivanova T, Petrenko A, Gritsko T, Vinokourova S, Eshilev E, Kobzeva V, Kisseljov F, Kisseljova N (2002) Methylation and silencing of the retinoic acid receptor-beta 2 gene in cervical cancer. BMC Cancer 21:2–4. doi:https://doi.org/10.1186/1471-2407-2-4Google Scholar
- Iyer NG, Ozdag H, Caldas C (2004) p300/CBP and cancer. Oncogene 23:4225–4231. doi:https://doi.org/10.1038/sj.onc.1207118PubMedGoogle Scholar
- Javier RT, Butel JS (2008) The history of tumor virology. Cancer Res 68:7693–7706. doi:https://doi.org/10.1158/0008-5472.CAN-08-3301PubMedPubMed CentralGoogle Scholar
- Jiang M, Abend JR, Johnson SF, Imperiale MJ (2009) The role of polyomaviruses in human disease. Virology 384:266–273. doi:https://doi.org/10.1016/j.virol.2008.09.027PubMedPubMed CentralGoogle Scholar
- Jones PA, Baylin SB (2002) The fundamental role of epigenetic events in cancer. Nat Rev Genet 3:415–428PubMedGoogle Scholar
- Jung JK, Arora P, Pagano JS, Jang KL (2007) Expression of DNA methyltransferase 1 is activated by hepatitis B virus X protein via a regulatory circuit involving the p16INK4a-cyclin D1-CDK 4/6-pRb-E2F1 pathway. Cancer Res 67:5771–5778. doi:https://doi.org/10.1158/0008-5472.CAN-07-0529PubMedGoogle Scholar
- Kalantari M, Calleja-Macias IE, Tewari D, Hagmar B, Lie K, Barrera-Saldana HA, Wiley DJ, Bernard HU (2004) Conserved methylation patterns of human papillomavirus type 16 DNA in asymptomatic infection and cervical neoplasia. J Virol 78(23):12762–12772. doi:https://doi.org/10.1128/JVI.78.23.12762-12772.2004PubMedPubMed CentralGoogle Scholar
- Kamoi K, Yamamoto K, Misawa A, Miyake A, Ishida T, Tanaka Y, Mochizuki M, Watanabe T (2006) SUV39H1 interacts with HTLV-1 Tax and abrogates Tax transactivation of HTLV-1 LTR. Retrovirology 3:5. doi:https://doi.org/10.1186/1742-4690-3-5PubMedPubMed CentralGoogle Scholar
- Kilger E, Kieser A, Baumann M, Hammerschmidt W (1998) Epstein–Barr virus-mediated B-cell proliferation is dependent upon latent membrane protein 1, which simulates an activated CD40 receptor. EMBO J 17(6):1700–1709. doi:https://doi.org/10.1093/emboj/17.6.1700PubMedPubMed CentralGoogle Scholar
- Kim K, Garner-Hamrick PA, Fisher C, Lee D, Lambert PF (2003) Methylation patterns of papillomavirus DNA, its influence on E2 function, and implications in viral infection. J Virol 77(23):12450–12459. doi:https://doi.org/10.1128/JVI.77.23.12450-12459.2003PubMedPubMed CentralGoogle Scholar
- Klein E, Kis LL, Klein G (2007) Epstein–Barr virus infection in humans: from harmless to life endangering virus–lymphocyte interactions. Oncogene 26:1297–1305. doi:https://doi.org/10.1038/sj.onc.1210240PubMedGoogle Scholar
- Knight JS, Lan K, Subramanian C, Robertson ES (2003) Epstein–Barr virus nuclear antigen 3C recruits histone deacetylase activity and associates with the corepressors mSin3A and NCoR in human B-cell lines. J Virol 77(7):4261–4272. doi:https://doi.org/10.1128/JVI.77.7.4261-4272.2003PubMedPubMed CentralGoogle Scholar
- Koiwa T, Hamano-Usami A, Ishida T, Okayama A, Yamaguchi K, Kamihira S, Watanabe T (2002) 5′-Long terminal repeat-selective CpG methylation of latent human T-cell leukemia virus type 1 provirus in vitro and in vivo. J Virol 76(18):9389–9397. doi:https://doi.org/10.1128/JVI.76.18.9389-9397.2002PubMedPubMed CentralGoogle Scholar
- Kosulin K, Haberler C, Hainfellner JA, Amann G, Lang S, Lion T (2007) Investigation of adenovirus occurrence in pediatric tumor entities. J Virol 81(14):7629–7635. doi:https://doi.org/10.1128/JVI.00355-07PubMedPubMed CentralGoogle Scholar
- Kulis M, Esteller M (2010) DNA methylation and cancer. Adv Genet 70:27–56. doi:https://doi.org/10.1016/B978-0-12-380866-0.60002-2PubMedGoogle Scholar
- Kuzmichev A, Margueron R, Vaquero A, Preissner TS, Scher M, Kirmizis A, Ouyang X, Brockdorff N, Abate-Shen C, Farnham P, Reinberg D (2005) Composition and histone substrates of polycomb repressive group complexes change during cellular differentiation. Proc Natl Acad Sci USA 102(6):1859–1864. doi:https://doi.org/10.1073/pnas.0409875102PubMedPubMed CentralGoogle Scholar
- Kwong J, Lo KW, To KF, Teo PM, Johnson PJ, Huang DP (2002) Promoter hypermethylation of multiple genes in nasopharyngeal carcinoma. Clin Cancer Res 8(1):131–137PubMedGoogle Scholar
- Lang SE, Hearing P (2003) The adenovirus E1A oncoprotein recruits the cellular TRRAP/GCN5 histone acetyltransferase complex. Oncogene 22:2836–2841. doi:https://doi.org/10.1038/sj.onc.1206376PubMedGoogle Scholar
- Li H, Minarovits J (2003) Host cell-dependent expression of latent Epstein–Barr virus genomes: regulation by DNA methylation. Adv Cancer Res 89:133–156. doi:https://doi.org/10.1016/S0065-230X(03)01004-2PubMedGoogle Scholar
- Li M, Damania B, Alvarez X, Ogryzko V, Ozato K, Jung JU (2000) Inhibition of p300 histone acetyltransferase by viral interferon regulatory factor. Mol Cell Biol 20:8254–8263. doi:https://doi.org/10.1128/MCB.20.21.8254-8263.2000PubMedPubMed CentralGoogle Scholar
- Lim C, Gwack Y, Hwang S, Kim S, Choe J (2001) The transcriptional activity of cAMP response element-binding protein-binding protein is modulated by the latency associated nuclear antigen of Kaposi’s sarcoma-associated herpesvirus. J Biol Chem 276(33):31016–31022. doi:https://doi.org/10.1074/jbc.M102431200PubMedGoogle Scholar
- Liu SS, Leung RC, Chan KY, Chiu PM, Cheung AN, Tam KF, Ng TY, Wong LC, Ngan HY (2004) p73 Expression is associated with the cellular radiosensitivity in cervical cancer after radiotherapy. Clin Cancer Res 10:3309–3316. doi:https://doi.org/10.1158/1078-0432.CCR-03-0119PubMedGoogle Scholar
- Lo KW, Kwong J, Hui AB, Chan SY, To KF, Chan AS, Chow LS, Teo PM, Johnson PJ, Huang DP (2001) High frequency of promoter hypermethylation of RASSF1A in nasopharyngeal carcinoma. Cancer Res 61(10):3877–3881PubMedGoogle Scholar
- Lo KW, Tsang YS, Kwong J, To KF, Teo PM, Huang DP (2002) Promoter hypermethylation of the EDNRB gene in nasopharyngeal carcinoma. Int J Cancer 98(5):651–655. doi:https://doi.org/10.1002/ijc.10271PubMedGoogle Scholar
- Longworth MS, Wilson R, Laimins LA (2005) HPV31 E7 facilitates replication by activating E2F2 transcription through its interaction with HDACs. EMBO J 24(10):1821–1823. doi:https://doi.org/10.1038/sj.emboj.7600651PubMedPubMed CentralGoogle Scholar
- Lu F, Day L, Gao SJ, Lieberman PM (2006) Acetylation of the latency-associated nuclear antigen regulates repression of Kaposi’s sarcoma-associated herpesvirus lytic transcription. J Virol 80:5273–5282. doi:https://doi.org/10.1128/JVI.02541-05PubMedPubMed CentralGoogle Scholar
- Martin ME, Berk AJ (1998) Adenovirus E1B 55K represses p53 activation in vitro. J Virol 72(4):3146–3154PubMedPubMed CentralGoogle Scholar
- Martin D, Gutkind JS (2008) Human tumor-associated viruses and new insights into the molecular mechanisms of cancer. Oncogene Suppl 2:S31–S42. doi:https://doi.org/10.1038/onc.2009.351Google Scholar
- Martinez-Garcia E, Licht JD (2010) Deregulation of H3K27 methylation in cancer. Nat Genet 42(2):100–101. doi:https://doi.org/10.1038/ng0210-100PubMedGoogle Scholar
- Matsuoka M, Jeang KT (2007) Human T-cell leukaemia virus type 1 (HTLV-1) infectivity and cellular transformation. Nat Rev Cancer 7:270–280. doi:https://doi.org/10.1038/nrc2111PubMedGoogle Scholar
- McCabe MT, Davis JN, Day ML (2005) Regulation of DNA methyltransferase 1 by the pRb/E2F1 pathway. Cancer Res 9:3624–3632. doi:https://doi.org/10.1158/0008-5472.CAN-04-2158Google Scholar
- McCabe MT, Low JA, Imperiale MJ, Day ML (2006) Human polyomavirus BKV transcriptionally activates DNA methyltransferase 1 through the pRb//E2F pathway. Oncogene 25:2727–2735. doi:https://doi.org/10.1038/sj.onc.1209266PubMedGoogle Scholar
- McLaughlin-Drubin ME, Munger K (2008) Viruses associated with human cancer. Biochim Biophys Acta 1782(3):127–150. doi:https://doi.org/10.1016/j.bbadis.2007.12.005PubMedPubMed CentralGoogle Scholar
- McLaughlin-Drubin ME, Crum CP, Münger K (2011) Human papillomavirus E7 oncoprotein induces KDM6A and KDM6B histone demethylase expression and causes epigenetic reprogramming. Proc Natl Acad Sci USA 108(5):2130–2135. doi:https://doi.org/10.1073/pnas.1009933108PubMedPubMed CentralGoogle Scholar
- Moens U (2009) Silencing viral microRNA as a novel antiviral therapy? J Biomed Biotechnol 2009:419539. doi:https://doi.org/10.1155/2009/419539PubMedPubMed CentralGoogle Scholar
- Mosialos G, Birkenbach M, Yalamanchili R, VanArsdale T, Ware C, Kieff E (1995) The Epstein–Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family. Cell 80(3):389–399. doi:https://doi.org/10.1016/0092-8674(95)90489-1PubMedGoogle Scholar
- Nair V, Zavolan M (2006) Virus-encoded microRNAs: novel regulators of gene expression. Trends Microbiol 14:169–175. doi:https://doi.org/10.1016/j.tim.2006.02.007PubMedGoogle Scholar
- Nakashima R, Fujita M, Enomoto T, Haba T, Yoshino K, Wada H, Kurachi H, Sasaki M, Wakasa K, Inoue M, Buzard G, Murata Y (1999a) Alteration of p16 and p15 genes in human uterine tumours. Br J Cancer 80(3–4):458–467. doi:https://doi.org/10.1038/sj.bjc.6690379PubMedPubMed CentralGoogle Scholar
- Nakashima R, Song H, Enomoto T, Murata Y, McClaid MR, Casto BC, Weghorst CM (1999b) Genetic alterations in the transforming growth factor receptor complex in sporadic endometrial carcinoma. Gene Expr 8(5–6):341–352PubMedGoogle Scholar
- Narayan G, Arias-Pulido H, Koul S, Vargas H, Zhang FF, Villella J, Schneider A, Terry MB, Mansukhani M, Murty VV (2003) Frequent promoter methylation of CDH1, DAPK, RARB, and HIC1 genes in carcinoma of cervix uteri: its relationship to clinical outcome. Mol Cancer 2:24. doi:https://doi.org/10.1186/1476-4598-2-24PubMedPubMed CentralGoogle Scholar
- Nevels M, Täuber B, Spruss T, Wolf H, Dobner T (2001) “Hit-and-run” transformation by adenovirus oncogenes. J Virol 75(7):3089–3094. doi:https://doi.org/10.1128/JVI.75.7.3089-3094.2001PubMedPubMed CentralGoogle Scholar
- Niemhom S, Kitazawa S, Kitazawa R, Maeda S, Leopairat J (2008) Hypermethylation of epithelial-cadherin gene promoter is associated with Epstein–Barr virus in nasopharyngeal carcinoma. Cancer Detect Prev 32:127–134. doi:https://doi.org/10.1016/j.cdp.2008.05.005PubMedGoogle Scholar
- Niller HH, Wolf H, Minarovits J (2008) Regulation and dysregulation of Epstein–Barr virus latency: implications for the development of autoimmune diseases. Autoimmunity 41:298–328. doi:https://doi.org/10.1080/08916930802024772PubMedGoogle Scholar
- Nuovo GJ, Plaia TW, Belinsky SA, Baylin SB, Herman JG (1999) In situ detection of the hypermethylation-induced inactivation of the p16 gene as an early event in oncogenesis. Proc Natl Acad Sci USA 96(22):12754–12759. doi:https://doi.org/10.1073/pnas.96.22.12754PubMedPubMed CentralGoogle Scholar
- Oliveira D (2007) DNA viruses in human cancer: an integrated overview on fundamental mechanisms of viral carcinogenesis. Cancer Lett 18;247(2):182–196. doi:https://doi.org/10.1016/j.canlet.2006.05.010Google Scholar
- O’Shea CC (2005) Viruses—seeking and destroying the tumor program. Oncogene 24(52):7640–7655. doi:https://doi.org/10.1038/sj.onc.1209047PubMedGoogle Scholar
- Pai S, O’Sullivan B, Abdul-Jabbar I, Peng J, Connoly G, Khanna R, Thomas R (2007) Nasopharyngeal carcinoma-associated Epstein–Barr virus-encoded oncogene latent membrane protein 1 potentiates regulatory T-cell function. Immunol Cell Biol 85(5):370–377. doi:https://doi.org/10.1038/sj.icb.7100046PubMedGoogle Scholar
- Pantry SN, Medveczky PG (2009) Epigenetic regulation of Kaposi’s sarcoma-associated herpesvirus replication. Semin Cancer Biol 19:153–157. doi:https://doi.org/10.1016/j.semcancer.2009.02.010PubMedPubMed CentralGoogle Scholar
- Park IY, Sohn BH, Yu E, Suh DJ, Chung YH, Lee JH, Surzycki SJ, Lee YI (2007a) Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein. Gastroenterology 132:1476–1494. doi:https://doi.org/10.1053/j.gastro.2007.01.034PubMedGoogle Scholar
- Park JH, Jeon JP, Shim SM, Nam HY, Kim JW, Han BG, Lee S (2007b) Wp specific methylation of highly proliferated LCLs. Biochem Biophys Res Commun 358:513–520. doi:https://doi.org/10.1016/j.bbrc.2007.04.169PubMedGoogle Scholar
- Patel D, Huang SM, Baglia LA, McCance DJ (1999) The E6 protein of human papillomavirus type 16 binds to and inhibits co-activation by CBP and p300. EMBO J 18(18):5061–5072. doi:https://doi.org/10.1093/emboj/18.18.5061PubMedPubMed CentralGoogle Scholar
- Paulson EJ, Speck SH (1999) Differential methylation of Epstein–Barr virus latency promoters facilitates viral persistence in healthy seropositive individuals. J Virol 73:9959–9968PubMedPubMed CentralGoogle Scholar
- Pfeffer S, Zavolan M, Grässer FA, Chien M, Russo JJ, Ju J, John B, Enright AJ, Marks D, Sander C, Tuschl T (2004) Identification of virus-encoded MicroRNAs. Science 304:734–736. doi:https://doi.org/10.1126/science.1096781PubMedGoogle Scholar
- Proietti FA, Carneiro-Proietti AB, Catalan-Soares BC, Murphy EL (2005) Global epidemiology of HTLV-I infection and associated diseases. Oncogene 24(39):6058–6068. doi:https://doi.org/10.1038/sj.onc.1208968PubMedGoogle Scholar
- Remus R, Kämmer C, Heller H, Schmitz B, Schell G, Doerfler W (1999) Insertion of foreign DNA into an established mammalian genome can alter the methylation of cellular DNA sequences. J Virol 73(2):1010–1022PubMedPubMed CentralGoogle Scholar
- Robertson KD (2001) DNA methylation, methyltransferases, and cancer. Oncogene 20:3139–3155. doi:https://doi.org/10.1038/sj.onc.1204341PubMedGoogle Scholar
- Robertson KD, Ambinder RF (1997) Methylation of the Epstein–Barr virus genome in normal lymphocytes. Blood 90:4480–4484PubMedGoogle Scholar
- Ropero S, Esteller M (2007) The role of histone deacetylases (HDACs) in human cancer. Mol Oncol 1(1):19–25. doi:https://doi.org/10.1016/j.molonc.2007.01.001PubMedGoogle Scholar
- Russell WC (2009) Adenoviruses: update on structure and function. J Gen Virol 90(Pt 1):1–20. doi:https://doi.org/10.1099/vir.0.003087-0PubMedGoogle Scholar
- Samols MA, Skalsky RL, Maldonado AM, Riva A, Lopez MC, Baker HV, Renne R (2007) Identification of cellular genes targeted by KSHV-encoded microRNAs. PLoS Pathog 3:e65. doi:https://doi.org/10.1371/journal.ppat.0030065PubMedPubMed CentralGoogle Scholar
- Seeger C, Mason WS (2000) Hepatitis B virus biology. Microbiol Mol Biol Rev 64:51–68. doi:https://doi.org/10.1128/MMBR.64.1.51-68.2000PubMedPubMed CentralGoogle Scholar
- Shamay M, Krithivas A, Zhang J, Hayward SD (2006) Recruitment of the de novo DNA methyltransferase Dnmt3a by Kaposi’s sarcoma-associated herpesvirus LANA. Proc Natl Acad Sci 103:14554–14559. doi:https://doi.org/10.1073/pnas.0604469103PubMedPubMed CentralGoogle Scholar
- Shivapurkar N, Toyooka S, Toyooka KO, Reddy J, Miyajima K, Suzuki M, Shigematsu H, Takahashi T, Parikh G, Pass HI, Chaudhary PM, Gazdar AF (2004) Aberrant methylation of trail decoy receptor genes is frequent in multiple tumor types. Int J Cancer 109(5):786–792. doi:https://doi.org/10.1002/ijc.20041PubMedGoogle Scholar
- Shon JK, Shon BH, Park IY, Lee SU, Fa L, Chang KY, Shin JH, Lee YI (2009) Hepatitis B virus-X protein recruits histone deacetylase 1 to repress insulin-like growth factor binding protein 3 transcription. Virus Res 139:14–21. doi:https://doi.org/10.1016/j.virusres.2008.09.006PubMedGoogle Scholar
- Simon JA, Lange CA (2008) Roles of the EZH2 histone methyltransferase in cancer epigenetics. Mutat Res 647(1–2):21–29. doi:https://doi.org/10.1016/j.mrfmmm.2008.07.010PubMedGoogle Scholar
- Soria C, Estermann FE, Espantman KC, O’Shea CC (2010) Heterochromatin silencing of p53 target genes by a small viral protein. Nature 466(7310):1076–1081. doi:https://doi.org/10.1038/nature09307PubMedPubMed CentralGoogle Scholar
- Sun R, Lin SF, Gradoville L, Yuan Y, Zhu F, Miller G (1998) A viral gene that activates lytic cycle expression of Kaposi’s sarcoma-associated herpesvirus. Proc Natl Acad Sci 95:10866–10871. doi:https://doi.org/10.1073/pnas.95.18.10866PubMedPubMed CentralGoogle Scholar
- Suzuki MM, Bird A (2008) DNA methylation landscapes: provocative insights from epigenomics. Nat Rev Genet 9(6):465–476. doi:https://doi.org/10.1038/nrg2341PubMedGoogle Scholar
- Szalmás A, Kónya J (2009) Epigenetic alterations in cervical carcinogenesis. Semin Cancer Biol 19(3):144–152. doi:https://doi.org/10.1016/j.semcancer.2009.02.011PubMedGoogle Scholar
- Takacs M, Banati F, Koroknai A, Segesdi J, Salamon D, Wolf H, Niller HH, Minarovits J (2010) Epigenetic regulation of latent Epstein–Barr virus promoters. Biochim Biophys Acta 1799(3–4):228–235. doi:https://doi.org/10.1016/j.bbagrm.2009.10.005PubMedGoogle Scholar
- Takeda S, Maeda M, Morikawa S, Taniguchi Y, Yasunaga J, Nosaka K, Tanaka Y, Matsuoka M (2004) Genetic and epigenetic inactivation of tax gene in adult T-cell leukemia cells. Int J Cancer 109(4):559–567. doi:https://doi.org/10.1002/ijc.20007PubMedGoogle Scholar
- Taniguchi Y, Nosaka K, Yasunaga J, Maeda M, Mueller N, Okayama A, Matsuoka M (2005) Silencing of human T-cell leukemia virus type I gene transcription by epigenetic mechanisms. Retrovirology 2:64. doi:https://doi.org/10.1186/1742-4690-2-64PubMedPubMed CentralGoogle Scholar
- Tao Q, Robertson KD (2003) Stealth technology: how Epstein–Barr virus utilizes DNA methylation to cloak itself from immune detection. Clin Immunol 109:53–63. doi:https://doi.org/10.1016/S1521-6616(03)00198-0PubMedGoogle Scholar
- Tao Q, Robertson KD, Manns A, Hildesheim A, Ambinder RF (1998) The Epstein–Barr virus major latent promoter Qp is constitutively active, hypomethylated, and methylation sensitive. J Virol 72:7075–7083PubMedPubMed CentralGoogle Scholar
- Täuber B, Dobner T (2001) Adenovirus early E4 genes in viral oncogenesis. Oncogene 20(54):7847–7854. doi:https://doi.org/10.1038/sj.onc.1204914PubMedGoogle Scholar
- Thain A, Jenkins O, Clarke AR, Gaston K (1996) CpG methylation directly inhibits binding of the human papillomavirus type 16 E2 protein to specific DNA sequences. J Virol 70(10):7233–7235PubMedPubMed CentralGoogle Scholar
- Tong JH, Tsang RK, Lo KW, Woo JK, Kwong J, Chan MW, Chang AR, van Hasselt CA, Huang DP, To KF (2002) Quantitative Epstein–Barr virus DNA analysis and detection of gene promoter hypermethylation in nasopharyngeal (NP) brushing samples from patients with NP carcinoma. Clin Cancer Res 8(8):2612–2619PubMedGoogle Scholar
- Tsai CN, Tsai CL, Tse KP, Chang HY, Chang YS (2002) The Epstein–Barr virus oncogene product, latent membrane protein 1, induces the downregulation of E-cadherin gene expression via activation of DNA methyltransferases. Proc Natl Acad Sci USA 99(15):10084–10089. doi:https://doi.org/10.1073/pnas.152059399PubMedPubMed CentralGoogle Scholar
- Turan T, Kalantari M, Calleja-Macias IE, Cubie HA, Cuschieri K, Villa LL, Skomedal H, Barrera-Saldaña HA, Bernard HU (2006) Methylation of the human papillomavirus-18 L1 gene: a biomarker of neoplastic progression? Virology 349(1):175–183. doi:https://doi.org/10.1016/j.virol.2005.12.033PubMedGoogle Scholar
- Turan T, Kalantari M, Cuschieri K, Cubie HA, Skomedal H, Bernard HU (2007) High-throughput detection of human papillomavirus-18 L1 gene methylation, a candidate biomarker for the progression of cervical neoplasia. Virology 361(1):185–193. doi:https://doi.org/10.1016/j.virol.2006.11.010PubMedPubMed CentralGoogle Scholar
- Van Tine BA, Dao LD, Wu SY, Sonbuchner TM, Lin BY, Zou N, Chiang CM, Broker TR, Chow LT (2004) Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning. Proc Natl Acad Sci USA 101(12):4030–4035. doi:https://doi.org/10.1073/pnas.0306848101PubMedPubMed CentralGoogle Scholar
- Virmani AK, Muller C, Rathi A, Zoechbauer-Mueller S, Mathis M, Gazdar AF (2001) Aberrant methylation during cervical carcinogenesis. Clin Cancer Res 7:584–589PubMedGoogle Scholar
- Wang L, Grossman SR, Kieff E (2000) Epstein–Barr virus nuclear protein 2 interacts with p300, CBP, and PCAF histone acetyltransferases inactivation of the LMP1 promoter. Proc Natl Acad Sci USA 97:430–435. doi:https://doi.org/10.1073/pnas.97.1.430PubMedPubMed CentralGoogle Scholar
- Whitby D (2009) Searching for targets of viral microRNAs. Nat Genet 41:7–8. doi:https://doi.org/10.1038/ng0109-7PubMedGoogle Scholar
- Widschwendter A, Gattringer C, Ivarsson L, Fiegl H, Schneitter A, Ramoni A, Müller HM, Wiedemair A, Jerabek S, Müller-Holzner E, Goebel G, Marth C, Widschwendter M (2004) Analysis of aberrant DNA methylation and human papillomavirus DNA in cervicovaginal specimens to detect invasive cervical cancer and its precursors. Clin Cancer Res 10(10):3396–3400. doi:https://doi.org/10.1158/1078-0432.CCR-03-0143PubMedGoogle Scholar
- Wiley DJ, Huh J, Rao JY, Chang C, Goetz M, Poulter M, Masongsong E, Chang CI, Bernard HU (2005) Methylation of human papillomavirus genomes in cells of anal epithelia of HIV-infected men. J Acquir Immune Defic Syndr 39(2):143–151PubMedGoogle Scholar
- Williams H, Crawford DH (2006) Epstein–Barr virus: the impact of scientific advances on clinical practice. Blood 107:862–869. doi:https://doi.org/10.1182/blood-2005-07-2702PubMedGoogle Scholar
- Woodman CB, Collins SI, Young LS (2007) The natural history of cervical HPV infection: unresolved issues. Nat Rev Cancer 7(1):11–22. doi:https://doi.org/10.1038/nrc2050PubMedGoogle Scholar
- Yokosuka O, Arai M (2006) Molecular biology of hepatitis B virus:effect of nucleotide substitutions on the clinical features of chronic hepatitis B. Med Mol Morphol 39:113–120. doi:https://doi.org/10.1007/s00795-006-0328-5PubMedGoogle Scholar
- Yoshida M (2001) Multiple viral strategies of HTLV-1 for dysregulation of cell growth control. Annu Rev Immunol 19:475–496. doi:https://doi.org/10.1146/annurev.immunol.19.1.475PubMedGoogle Scholar
- Yoshida M (2005) Discovery of HTLV-1, the first human retrovirus, its unique regulatory mechanisms, and insights into pathogenesis. Oncogene 24(39):5931–5937. doi:https://doi.org/10.1038/sj.onc.1208981PubMedGoogle Scholar
- Yoshioka M, Kikuta H, Ishiguro N, Endo R, Kobayashi K (2003) Latency pattern of Epstein–Barr virus and methylation status in Epstein–Barr virus-associated hemophagocytic syndrome. J Med Virol 70:410–419. doi:https://doi.org/10.1002/jmv.10411PubMedGoogle Scholar
- Young LS, Rickinson AB (2004) Epstein–Barr virus: 40 years on. Nat Rev Cancer 4:757–768. doi:https://doi.org/10.1038/nrc1452PubMedGoogle Scholar
- Yu MY, Tong JH, Chan PK, Lee TL, Chan MW, Chan AW, Lo KW, To KF (2003) Hypermethylation of the tumor suppressor gene RASSFIA and frequent concomitant loss of heterozygosity at 3p21in cervical cancers. Int J Cancer 105:204–209. doi:https://doi.org/10.1002/ijc.11051PubMedGoogle Scholar
- Zambrano P, Segura-Pacheco B, Pérez-Cárdenas E, Cetina L, Revilla-Vázquez A, Taja-Chayeb L, Chávez-Blanco A, Angeles E, Cabrera G, Sandoval K, Trejo-Becerril C, Chanona-Vilchis J, Dueńas-González A (2005) A phase I study of hydralazine to demethylate and reactivate the expression of tumor suppressor genes. BMC Cancer 5:44. doi:https://doi.org/10.1186/1471-2407-5-44PubMedPubMed CentralGoogle Scholar
- Zhang B, Laribee RN, Klemsz MJ, Roman A (2004) Human papillomavirus type 16 E7 protein increases acetylation of histone H3 in human foreskin keratinocytes. Virology 329(1):189–198. doi:https://doi.org/10.1016/j.virol.2004.08.009PubMedGoogle Scholar
- Zheng ZM (2010) Viral oncogenes, noncoding RNAs, and RNA splicing in human tumor viruses. Int J Biol Sci 6(7):730–755PubMedPubMed CentralGoogle Scholar
- Zheng DL, Zhang L, Cheng N, Xu X, Deng Q, Teng XM, Wang KS, Zhang X, Huang J, Han ZG (2009) Epigenetic modification induced by hepatitis B virus X protein via interaction with de novo DNA methyltransferase DNMT3A. J Hepatol 50:377–387. doi:https://doi.org/10.1016/j.jhep.2008.10.019PubMedGoogle Scholar
- Zimmermann H, Degenkolbe R, Bernard HU, O’Connor MJ (1999) The human papillomavirus type 16 E6 oncoprotein can down-regulate p53 activity by targeting the transcriptional coactivator CBP/p300. J Virol 73(8):6209–6219PubMedPubMed CentralGoogle Scholar
- zur Hausen H (2006) Infections causing human cancer. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, GermanyGoogle Scholar
- zur Hausen H (2008) Novel human polyomaviruses—re-emergence of a well known virus family as possible human carcinogens. Int J Cancer 123:247–250. doi:https://doi.org/10.1002/ijc.23620PubMedGoogle Scholar
- zur Hausen H (2009) Papillomaviruses in the causation of human cancers—a brief historical account. Virology 384(2):260–265. doi:https://doi.org/10.1016/j.virol.2008.11.046PubMedGoogle Scholar