- Letter to the Editor
- Open Access
Loss of nuclear localization of TET2 in colorectal cancer
Clinical Epigeneticsvolume 8, Article number: 9 (2016)
5-Hydroxymethylcytosine (5hmC) is lost in multiple human cancers, including colorectal cancer (CRC). Decreased ten-eleven translocation 1 (TET1) messenger RNA (mRNA), but not other two TET family members, has been observed in the colorectal cancer and is crucial for colorectal cancer initiation. Here, we show that nuclear localization of TET2 was lost in a significant portion of CRC tissues, in association with metastasis. In CRC cells, nuclear expression of TET2 were absent but not TET3. Nuclear export inhibitor can increase the 5hmC level in CRC cells, probably through regulating TET2. Our results indicate a new mechanism of TET2 dysregulation in colorectal cancer.
5-Hydroxymethylcytosine (5hmC) is lost in multiple human cancers, including colorectal cancer. Inactivation of the ten-eleven translocation (TET) family members, the DNA hydroxylases catalyze 5mC into 5hmC, was closely related to the cancer initiation and progression. Recently, F Neri et al. reported the downregulation of TET1 messenger RNA (mRNA) expression level, but not other two TET family members, in colon cancer and showed that decreased TET1 mRNA was crucial for colon cancer initiation and TET1 functioned as a tumor suppressor by inhibiting the WNT pathway . However, another group observed the decreased mRNA level of all TET family members in the colorectal cancer . These contradictory findings could be due to the interfering by the stromal and infiltrating immune cells in the tumor tissues, the unstable mRNA in the tissue samples, and the RNA degradation during the RNA extraction. Besides, it was reported that the reduced levels of 5hmC in colorectal cancers is not correlated with TET mRNA levels , which indicates that dysregulation of TET protein could play a vital role in colorectal cancer. Thus, we analyzed both the mRNA and protein level of the TET family members in our colorectal cancer specimens.
Consistent with the finding of F Neri and another previous report , only TET1 was significantly decreased in the colorectal cancer tissues and cell lines at mRNA level (Additional file 1: Figure S1A and B). No detectable TET1 protein was observed in the CRC cell lines with low TET1 mRNA level (Additional file 1: Figure S1C), indicating that the decreased TET1 mRNA level resulted in low TET1 protein level in colorectal cancer. After testing several commercial TET2 antibodies, TET2 antibody (ab94580) was used for the immunohistochemical analysis of a CRC tissue array for the high sensitivity and specificity of this antibody (Additional file 1: Figure S1D). As shown in Fig. 1a, while normal and some CRC tissues contain positive nuclear staining of TET2, interestingly, a large portion of CRC tissues showed loss of nuclear staining of TET2 (Fig. 1b). After comparing TET2 nuclear expression with clinical data, we found that loss of nuclear TET2 expression was correlated with a more aggressive distal metastasis phenotype (P = 0.021) (Additional file 2: Table S1). No difference in the protein expression level and localization of TET3 between the normal and cancer tissues was observed (Additional file 1: Figure S1E). To confirm the finding of immunohistochemical (IHC) analysis, we detected the TET2 protein expression in the CRC cell lines. Consistent to IHC data, nuclear localization of TET2 was lost in 5 CRC cell lines, 1 lung cancer cell line, and 293T cells we tested (Fig. 1c). However, strong nuclear localization of TET3, another TET family member expressed in CRC cells, was detected in these cell lines. Also, we observed increased 5hmC level in multiple CRC cell lines after treatment with the nuclear export inhibitor, leptomycin B (LMB) (Fig. 1d). Knockdown TET2 further demonstrated that increasing 5hmC induced by LMB treatment was due to the TET2 protein (Fig. 1e).
DNA methylation is a therapeutic target for cancer treatment . DNA hypermethylation occurs to the promoter of tumor suppressor genes, resulting in decreased expression of the tumor suppressor and cancer initiation and progression . Inhibitors of DNA methyltransferases like 5-azacytidine and 5-aza-2′-deoxycytidine have been shown the efficacy in the treatment of multiple cancers to induce the expression of the tumor suppressor genes . DNA hypermethylation of tumor suppressor genes could be due to the loss of active DNA demethylation which is consistent with global loss of 5hmC level in cancers [4, 7]. Thus, re-activation of the active DNA demethylation process could also lead to the re-expression of the tumor suppressor genes. The nuclear export inhibitors is also a promising cancer treatment drugs which have been shown to inhibit nuclear export of tumor suppressors like TP53 . Here, we showed that LMB treatment can increase the global level of 5hmC probably through regulating TET2 in the CRC cell lines, which could promote the active demethylation and expression of tumor suppressor genes. Our results provided a novel mechanism that nuclear export inhibitor functioning through restoring the 5hmC level and potential expression of tumor suppressor genes which need to be further studied in the future.
Several mechanisms have been reported for the dysregulation of TET2 in cancers, including mutation of TET2 gene  and decreasing TET2 mRNA expression level [10, 11]. Nuclear exclusion of TET1 protein was found in the IDH1 wild-type gliomas . Nuclear translocation of TET2 was first reported in the B cells ; furthermore, our results demonstrated that nuclear translocation of TET2 was also dysregulated in colorectal cancer, probably through the post-translational modification. Since TET2 needs to interact with DNA binding partners to regulate gene expression in the nucleus, loss of TET2 nuclear expression impaired TET2’s function. Thus, TET2 could also be a tumor suppressor in colorectal cancer, and the tumor suppressor function of both TET1 and TET2 was impaired in colorectal cancer through different mechanism.
Neri F, Dettori D, Incarnato D, Krepelova A, Rapelli S, Maldotti M, et al. TET1 is a tumour suppressor that inhibits colon cancer growth by derepressing inhibitors of the WNT pathway. Oncogene. 2015;34(32):4168–76.
Rawluszko-Wieczorek AA, Siera A, Horbacka K, Horst N, Krokowicz P, Jagodzinski PP. Clinical significance of DNA methylation mRNA levels of TET family members in colorectal cancer. J Cancer Res Clin Oncol. 2015;141(8):1379–92.
Uribe-Lewis S, Stark R, Carroll T, Dunning MJ, Bachman M, Ito Y, et al. 5-hydroxymethylcytosine marks promoters in colon that resist DNA hypermethylation in cancer. Genome Biol. 2015;16:69.
Kudo Y, Tateishi K, Yamamoto K, Yamamoto S, Asaoka Y, Ijichi H, et al. Loss of 5-hydroxymethylcytosine is accompanied with malignant cellular transformation. Cancer Sci. 2012;103(4):670–6.
Szyf M. Epigenetics, DNA methylation, and chromatin modifying drugs. Annu Rev Pharmacol Toxicol. 2009;49:243–63.
Heyn H, Esteller M. DNA methylation profiling in the clinic: applications and challenges. Nat Rev Genet. 2012;13(10):679–92.
Jin SG, Jiang Y, Qiu R, Rauch TA, Wang Y, Schackert G, et al. 5-Hydroxymethylcytosine is strongly depleted in human cancers but its levels do not correlate with IDH1 mutations. Cancer Res. 2011;71(24):7360–5.
Mutka SC, Yang WQ, Dong SD, Ward SL, Craig DA, Timmermans PB, et al. Identification of nuclear export inhibitors with potent anticancer activity in vivo. Cancer Res. 2009;69(2):510–7.
Ko M, Huang Y, Jankowska AM, Pape UJ, Tahiliani M, Bandukwala HS, et al. Impaired hydroxylation of 5-methylcytosine in myeloid cancers with mutant TET2. Nature. 2010;468(7325):839–43.
Yang H, Liu Y, Bai F, Zhang JY, Ma SH, Liu J, et al. Tumor development is associated with decrease of TET gene expression and 5-methylcytosine hydroxylation. Oncogene. 2013;32(5):663–9.
Sajadian SO, Ehnert S, Vakilian H, Koutsouraki E, Damm G, Seehofer D, et al. Induction of active demethylation and 5hmC formation by 5-azacytidine is TET2 dependent and suggests new treatment strategies against hepatocellular carcinoma. Clin Epigenetics. 2015;7(1):98.
Muller T, Gessi M, Waha A, Isselstein LJ, Luxen D, Freihoff D, et al. Nuclear exclusion of TET1 is associated with loss of 5-hydroxymethylcytosine in IDH1 wild-type gliomas. Am J Pathol. 2012;181(2):675–83.
Di Stefano B, Sardina JL, van Oevelen C, Collombet S, Kallin EM, Vicent GP, et al. C/EBPalpha poises B cells for rapid reprogramming into induced pluripotent stem cells. Nature. 2014;506(7487):235–9.
This work was supported by the National Natural Science Foundation of China (Grant No. 81372636, No. 81302089, No. 81572378, No. 81502020), the National High Technology Research and Development Program of China (863 Program) (Grant No. 2014AA020801), and the Shanghai Excellent Young Teachers Program (Grant No. ZZjdyx13074).
The authors declare that they have no competing interests.
HY performed the cellular experiments with the help of LZ. WG performed the immunohistochemical analysis of TET2 in the CRC tissues and clinicopathological analysis (Additional file 3). LCY and CL designed the project. LCY, CL, and YY wrote the manuscript. All authors read and approved the final manuscript.
Yuji Huang and Guanghui Wang contributed equally to this work.
(A) The mRNA expression level of TET family members in 9 paired normal mucosa tissues and colorectal cancer tissues were detected by qPCR. (B) The mRNA expression level of TET family members in normal colon cell line (CCD-841) and six colorectal cancer cell lines were detected by qPCR. (C) The TET1 protein expression level in the colorectal cancer cell lines were detected by western blot. (D) Western blot analysis of several TET2 antibodies showed that the TET2 antibody (ab94580) used for IHC was specific for TET2 protein. FLAG-Tet2 was transfected into 293T cells by Lipofectamine 2000. 36h later, cells were lysed and detected by using FLAG antibody and TET2 antibody. (E) Immunohistochemical analysis of TET3 in colorectal cancer samples and normal mucosa tissues. Representative images of normal mucosa tissue and colorectal cancer tissue were shown. Scale bar: 100 μm. (PDF 419 kb)
Correlation of TET2 cytoplasmic expression with CRC patients’ pathological features. (DOC 84 kb)
Supplementary materials and methods. (DOCX 22 kb)