Fig. 5
From: Epigenetic tumor heterogeneity in the era of single-cell profiling with nanopore sequencing
Biological nanopore instruments with representative ssDNA and protein-bound DNA events [67, 93]. Image reprinted from [67] with permission of the publisher (CCC License ID: 600061571, 25 Nov 2021). A The nanopore instrument uses an amplifier to apply a command voltage Vc and measure ionic current Ip through the nanopore channel. B At 120Â mV in 1Â M buffered KCl solution, 120 pA of open-channel current is attenuated to 15 pA for 0.2Â ms upon the capture of ssDNA into the channel from the cis-chamber until the DNA passes through the pore. With Exol and ssDNA in the cis-chamber, bound events are also observed in which the duration of the current shift is extended 2Â ms [67]