Methylation site | Before dexamethasone (%) | After dexamethasone (%) | P value |
---|
P1 promoter CpG | 32 (29 to 35) | 30 (28 to 33) | 0.026 |
P2 promoter (CpG mean) | 42 (37 to 43) | 38 (36 to 41) | 0.093 |
(P2_CpG 2) | 22 (16 to 24) | 18 (17 to 22) | 0.072 |
(P2 _CpG 3) | 41 (33 to 44) | 37 (35 to 42) | 0.409 |
(P2 _CpG 4) | 63 (58 to 66) | 59 (56 to 62) | 0.065 |
- Data presented as median (25th to 75th percentile). Methylation scores (approximating % of alleles with cytosine methylation at specific CpG sites within a tissue sample) were obtained from the Mass Spec output using EpiTYPER v1.0.5 software (Sequenom Inc, San Diego, CA, USA). Only a single CpG site was present in the P1 promoter region tested, whereas three sites in close proximity were assessed in P2.