Fig. 4
Detection of cervical cancer and high-grade lesions in cervical smears. Quantitative Multiplex-Methylation-Specific PCR (QM-MSP) was performed on cervical smears (N = 244 total) from the USA (N = 77), Vietnam (N = 117), and S. Africa (N = 50) and data were pooled for analyses. A Histogram indicates the magnitude of Cumulative Methylation-5 (CM-5) (Y-axis) for each sample (X-axis). B Box and whisker plot shows comparison of CM-5 in samples of cervical smears from normal (N), low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma (SCC) (P < 0.0001, Mann–Whitney). C Receiver operator curve area under the curve (ROC AUC) results are shown. Sensitivity and specificity were based on the 95th percentile of CM-5 in normal samples (dotted line in histogram)