Fig. 1

The information of CpG methylation sites in primer DIO3-FA27 and the target fragment sequence in the DIO3 promoter region (A The dots represent the CpG sites to be tested in the target fragment; B The position of the amplified segment of interest on the chromosome: chr14: 101561179–101561497 and the DIO3-FA27 target fragment contained 28 CpGs: the blue bases represent CpG sites to be tested; the red base represents the CpG site that was not detected)