Fig. 3

Comparison of self-renewal and tumor growth between H460 or A549 cells with the corresponding LCSLCs A DNMT1 activity; B DNMT1 mRNA and protein expression; C miR-152-3p levels; D miR-152-3p promoter methylation in H460 or A549 cells and their corresponding LCSLCs; E SOS1 mRNA and protein expression; F, G A549 and H460 cells exhibit similarities with their corresponding LCSLCs in terms of both sphere and colony formation rates (scale bar, 100 μm); H, I CD44, CD133, Oct4, and Sox2 protein expression. All experiments were conducted in triplicate. *p < 0.05 vs. A549 or H460 cells. The in vivo tumorigenicity of H460 tumorigenic cells and their LCSLCs J, including tumor-initiating cell frequency K, histology, DNMT1, SOS1, CD44, and miR-152-3p expression (scale bar, 50 μm) L. Data were obtained from xenografts at six vaccination sites (n = 6 graft tumors)