Fig. 4.

182KO ALL cells are enriched more LIC (B220⁺CD43⁺) cells. A The percentage of LIC was measured in BM GFP⁺ cells from 182WT and 182KO ALL mice. Representative flow plots (left) and statistical analysis of the percentage of LIC (right) are shown. B Edu staining for BM GFP⁺ cells from 182WT and 182KO ALL mice. Shown are the representative flow plots (left) and statistical analysis of the percentage of Edu⁺ cells (right). C–E BM GFP⁺ cells were sorted from 182WT and 182KO ALL mice and plated on murine methylcellulose medium (8000/dish). Colony number (D) was counted after ten days of culture, and total cell number (E) was measured after colonies were washed. The Bar represent 10 μm. F and G OS was counted in primary (n = 7 for WT and KO mice) and secondary BMT (n = 5 for WT and KO mice). H Limiting dilution assay of BM GFP⁺ cells from secondary BMT leukemic mice with 182WT (n = 7) and 182KO (n = 7). The frequency of LSC and P value were determined via L-calc software. *P < 0.05; **P < 0.01; ***P < 0.001