Fig. 6

The effect of SAH and CHIR in osteoblasts and periodontitis mice. A, B Representative micro-CT reconstruction images of the maxillae. Red area showed the CEJ-ABC distance. Red lines showed the alveolar bone. CEJ, cementum enamel junction; ABC, alveolar bone crest. C H&E staining images of the periodontal tissues. Black arrows point to ABC. Black lines represent the bone loss in root furcation. D TRAP staining images in root furcation. R, root. E, F IHC staining of RUNX2 and c-MYC in the periodontal ligament and alveolar bone. G The expression of c-Myc, 45S rRNA, RANKL(Tnfsf11) and TNF-α(Tnfa) of periodontal tissues was detected by qRT-PCR. n = 3. H Schematic diagram illustrating the mechanism of METTL3 regulating osteoblast ribosome biogenesis and oxidative phosphorylation via Wnt/β-catenin/c-Myc signaling in periodontitis. All data represent the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001