Fig. 5
From: METTL3 promotes osteoblast ribosome biogenesis and alleviates periodontitis
The effect of METTL3 and YTHDF2 on the mRNA stability of Dkk3 and Sostdc1 in Wnt/β-catenin signaling. A GSEA analysis identified the negative regulation of Wnt signaling pathway. B, D, H The METTL3/YTHDF2-kncokdown cells were stimulated with LPS and osteogenic induction medium for 64 h and then treated with 5 μg/mL ActD for 0–8 h. The mRNA expression of Dact3, Hic1, Lzts2, Dkk3 and Sostdc1 was measured by qRT-PCR. n = 3. C, G The expression of Dkk3 and Sostdc1 was examined by qRT-PCR during 3-day LPS-stimulated osteoblast differentiation. n = 4. E The binding between YTHDF2 and Dkk3 or Sostdc1 was determined by RIP assay. n = 3. F The knockdown efficiency was verified by western blotting. The results are shown as the mean ± SD. *P < 0.05; **P < 0.01