Fig. 4

The role of Wnt/β-catenin/c-Myc signaling in METTL3-depletion cells. A The expression and mRNA degradation of c-Myc were tested by qRT-PCR. n = 3. B The protein level of β-catenin and c-Myc on day 3 was measured by western blotting. C–F The cells were treated with or without CHIR for 3 days. The expression of β-catenin, c-Myc, rRNAs and RPs was evaluated by qRT-PCR and western blotting. c-Myc, rRNAs, n = 4. RPs, n = 3. G, H The ribosomal level and neo protein synthesis rate were measured on day 3. n = 3. I, J The ATP level and MMP was detected on day 3. n = 3. (K) The osteogenic markers RUNX2, OSX, and OCN were measured by western blotting. All data represent the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001