Fig. 3

The effect of METTL3 on oxidative phosphorylation and osteoblast differentiation. A GSEA analysis found that METTL3 depletion affected the genes in the ATP biosynthetic process and oxidative phosphorylation. B The ATP level was assessed in LPS-treated osteoblasts after METTL3 knockdown and overexpression. n = 3. C The expression of respiratory chain genes was detected at day 3. Knockdown, n = 4. Overexpression, n = 3. D The level of MMP was measured after METTL3 knockdown. n = 3. E–H MC3T3-E1 cells were treated by osteogenic induction medium and LPS with or without 100 nM CX-5461/rotenone. Cell proliferation was analyzed by CCK8 assay (E). The expression level of RUNX2 and OSX on day 3 was measured by western blotting (F) and qRT-PCR (G). The ALP staining was performed on day 7 (H). n = 3. All data represent the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001