Fig. 2
From: METTL3 promotes osteoblast ribosome biogenesis and alleviates periodontitis
The effect of METTL3 knockdown and overexpression on ribosome biogenesis. A, C The expression of rRNAs and RPs was detected in METTL3 knockdown cells after LPS and osteogenic induction for 3 days. rRNAs, n = 4. RPs, n = 3. B A heatmap of differentially expressed RP genes. D The ribosome fractions were separated and quantitated by absorbance at 260 nm. The translation efficiency was recorded as the ratio between the absorbance of polysomes and not translating monosomes. E The protein synthesis rate was measured by puromycin incorporation assay in siMETTL3 cells. n = 3. F The overexpressing efficiency of METTL3 was tested by western blotting. G The expression of rRNAs and RPs was evaluated in METTL3-overexpressed cells. n = 3. All data represent the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001