Fig. 1

Identified of ribosome as METTL3 target in RNA-seq analysis in LPS-stimulated osteoblasts. A The knockdown efficiency was verified by western blotting. B KEGG pathway and GO enrichment analysis shown the differentially expressed transcripts between shCTRL and shMETTL3 group. C The GSEA enrichment plot of the gene sets. D The rRNAs expressions were detected after osteogenic and LPS stimulation 3 days. n = 4. GM, growth medium; OM, osteogenic induction medium. E The cells were treated with 5 μg/mL puromycin for 40 min. The nascent polypeptides were measured by western blotting. β-Actin was used as internal control. n = 3. All data represent the mean ± SD. *P < 0.05