From: Degradation of methylation signals in cryopreserved DNA
Term | Discovery cohort (n = 126) | |||||
---|---|---|---|---|---|---|
Reduced model | Full model | |||||
Estimate (95% CI) | p-value | Estimate (95% CI) | p-value | |||
Intercept | 0.29 | (0.22–0.37) | 7.5e − 12* | 4.58 | (3.59 – 5.58) | 4.6e − 15 |
Storage as DNA | 9.64e-03 | (− 1.09e − 02–3.02e − 02) | 0.360 | 8.57e − 03 | (− 7.29e − 03–2.44e − 02) | 0.292 |
Storage as buffy coat | − 2.52e − 06 | (− 3.52e − 04–3.47e − 04) | 0.989 | |||
Age | 2.31e − 07 | (− 2.17e − 06–2.63e − 06) | 0.851 | |||
CD8 + T | − 4.28 | (− 5.29– − 3.27) | 1.8e − 13 | |||
CD4 + T | − 4.15 | (− 5.20– − 3.09) | 5.4e − 12 | |||
NK cell | − 4.42 | (− 5.40– − 3.45) | 9.1e − 15 | |||
B cell | − 4.24 | (− 5.25– − 3.23) | 3.3e − 13 | |||
Monocytes | − 4.48 | (− 5.55– − 3.42) | 2.9e − 13 | |||
Granulocytes | − 4.34 | (− 5.35– − 3.33) | 1.3e − 13 | |||
Model AIC | − 420.3 | − 488.8 | ||||
Model BIC | − 411.8 | − 457.6 |
Term | Validation cohort (n = 136) | |||||
---|---|---|---|---|---|---|
Reduced model | Full model | |||||
Estimate (95% CI) | p-value | Estimate (95% CI) | p-value | |||
Intercept | 0.28 | (0.25–0.31) | 2.8e − 36 | 3.89 | (2.80–4.98) | 1.5e − 10 |
Storage as DNA | 0.02 | (0.00–0.03) | 0.030 | 9.36e − 03 | (− 3.01e − 03–2.17e − 02) | 0.140 |
Age | 1.42e − 06 | (-1.64e − 06–4.48e − 06) | 0.366 | |||
CD8 + T | − 3.65 | (− 4.76– − 2.55) | 2.0e − 09 | |||
CD4 + T | − 3.47 | (− 4.61– − 2.32) | 2.5e − 08 | |||
NK cell | − 3.60 | (− 4.68– − 2.51) | 1.6e − 09 | |||
B cell | − 3.38 | (− 4.51– − 2.25) | 4.0e − 08 | |||
Monocytes | − 3.48 | (− 4.63– − 2.33) | 2.7e − 08 | |||
Granulocytes | − 3.70 | (− 4.80– − 2.60) | 1.0e − 09 | |||
Model AIC | − 494.7 | − 558.0 | ||||
Model BIC | − 486.0 | − 528.9 |