Fig. 2

Landscape of DNA methylation of adenoma, carcinoma, and normal colorectal tissues in EPIC datasets. A Density plot showing the characteristic bimodal distribution of methylation β-values in all 3 tissues based on EPIC array data. B Violin plot of the mean methylation in each of the tissues, shows overall methylation decreases with increase in malignancy. C MDS plot highlighting the data structure and sample relationship among the tissue groups in EPIC array data. D tSNE plot showing a defined cluster for each of the different tissues, highlighting the ability to resolve samples based on their methylation patterns, despite overlap between adenomas and carcinomas. MDS multidimensional scaling, tSNE t-distributed stochastic neighbor embedding, A adenoma, C carcinoma, N normal tissue