From: Promoter methylation changes and vascular dysfunction in pre-eclamptic umbilical vein
Primer | Nucleotide Sequence (5′ to 3′) | |
---|---|---|
Sense | Anti-sense | |
qRT-PCR primers | ||
 AVPR1a | TCGTGACGGCTTACATCGTC | GAGTCTTGAAGGAGATGGCCA |
 AVPR1b | CCTCATCTGCCATGAGATCTG | GCCACATTGGTGGAATCTTCATCA |
 AVPR2 | TGACGCTAGTGATTGTGGTC | GACACGCTGCTGCTGAAAGA |
 OTXR | TCAGCAGCGTCAAGCTCATC | GTGAACAGCATGTAGATCCAG |
 PKCα | CTCTGCGGAATGGATCACACT | GGACTCATTCCACTGCGGAT |
 PKCβ | GACCTCATGTATCACATCCAG | GAGTGCCACAGAATGTCTTG |
 PKCδ | TCCAAGGACATCCTGGAGAAG | GTCTCTGGGTGACTTCACTT |
 PKCε | TACAAGGTCCCTACCTTCTG | TCGGCCAGTACTTTGGCGAT |
 PKCγ | TGCCTGTGCCCGTCATATCCT | AGAGTCCAGAACGCTAAGGT |
Bisulfite sequencing primers | ||
 AVPR1a | AGAGTTAGGTTTAGGTGTAGGAGTTAGATG | CAAATTCCTCCTCACAATAAATAAAATC |
 OTXR | TTTYGTTTYGGAGGGGTTTG | AATACTAAACTAAAATCTCTCACTAAAACCTC |
 PKCβ-1 | GGTAGTAGTTGGGYGAGTGATAgttt | ACCCCRCAACCRAATCAAC |
 PKCβ-2 | GgtttYGgggtYGgtATTTTT | CTCACCAAATAAAATCRATACAATAACTACAAA |