Fig. 3

The effects of DIRAS1 on colorectal cancer cell proliferation, migration, invasion, and apoptosis in DIRAS1 re-expressed colorectal cancer cells. a Growth curves represent cell viability analyzed by the MTT assay in DIRAS1 re-expressed and unexpressed DLD1 and RKO cells. The experiments were performed in triplicate. (**P < 0.01). b Colony formation assays show colony numbers in DLD1 and RKO cells before and after re-expression of DIRAS1. Each experiment was repeated three times. The average number of tumor clones is represented by bar diagram. (* P < 0.05, ** P < 0.01). c Cell migration and invasion results in DLD1 and RKO cells before and after re-expression of DIRAS1, d ratios are presented by bar diagram. Each experiment was repeated three times. (**P < 0.01). e The expression levels of DIRAS1, MMP2, and MMP9 were detected by Western blot in DIRAS1 unexpressed and re-expressed DLD1 and RKO cells. f, g Representative images of Annexin-V and PI double staining in DIRAS1 unexpressed and re-expressed colorectal cancer cells (*P < 0.05). h Western blots show the levels of cleaved caspase-3 in DIRAS1 re-expressed and unexpressed colorectal cancer cells. The levels of cleaved caspase-3 were increased in DIRAS1 re-expressed DLD1 and RKO cells