Fig. 2

Resolution and identification of purified histones from paired serum and liver tissue of normal and tumour. a Silver stained 18% SDS-PAGE confirmed the integrity of the histones isolated from serum samples (1 and 2) of HCC-harboring rats by DAE protocol. All the core histones, H3, H2A, H2B and H4 are marked with an arrow, whereas the star marks high molecular weight proteins. b H&E-stained section of normal liver and HCC showing the altered histology in HCC at 20× and 40× magnification. c Silver-stained 18% SDS-PAGE showing the integrity of the purified histones loaded in increasing volumes (5, 10 and 15 μl) (i) samples of normal and tumour tissues and (ii) serum of normal and tumour bearing rats by the DAE protocol. The core histones H2A, H2B, H3 and H4 are marked. d Histones isolated from tissues and serum of both normal and tumour harboring rats are subjected to LC-MS after trypsin digestion. The obtained data on number of peptides of histones identified along with sequence coverage are tabulated and confirmed the identity of purified proteins by DAE method. H&E haematoxylin and eosin, NDEA N-nitrosodiethylamine