Fig. 3

Analysis of cell death induced by glucose deprivation or etoposide treatment in cells with JMJD6 knock-down. a Myc83-derived cell lines (parental line from a MMTV-Myc tumor) with stable expression of two independent shRNAs targeting JMJD6 or with empty vector (EV) control were treated with 100 μM etoposide or grown in glucose-free media for 20 h. Cell death was measured using CytoTox-Glo reagent. The experiments were repeated 3 times and the percentage of dead cells in each experiment was expressed relative to control (EV) cells. b NMuMG cells with MycER™ and shJMJD6 expression were first treated with 150 nM 4-OHT (MycON) or ethanol (MycOFF) for 24 h to activate Myc and then treated as in a Black bars—MycOFF (ethanol-treated cells), open bars—MycON (4-OHT-treated cells). Results were normalized to EV control with MycOFF. *p < 0.05, **p < 0.01