Figure 1

Genomic location of MS-MLPA ICR1 sites M1 to M5 and of CTCF binding sites 1 to 7. Genomic position (upright numbers) of CpG sites M1-5 are shown according to basic local alignment search tool (BLAST) search with the relevant sequences provided by the manufacturer (see also Additional file 1). Exon sequences were obtained from NM_000612.4 (IGF2) and NR_002196.1 (H19) at NCBI Nucleotide and matched against human genome build GRCh37 from the Genome Reference Consortium accessible at http://www.ensembl.org. CCCTC-binding factor (CTCF) site sequences from Bell et al. [22] were matched to the genomic sequence by BLAST search (Additional file 1). Figure adapted and modified from Kannenberg et al. [31]. MS-MLPA, methylation-specific multiple ligation-dependent probe amplification.