Fig. 3
Technical validation of the 5-marker panel in archival tissue. Quantitative Multiplex-Methylation-Specific PCR (QM-MSP) was performed on FFPE tissue sections from (A) the USA (N = 63), B S. Africa (N = 69), and C Vietnam (N = 120). The histogram bar indicates the magnitude of Cumulative Methylation-5 (CM-5) (Y-axis) in each sample (X-axis). Box and whisker plots in A, B, and C show comparisons of CM-5 between groups as indicated. Receiver operator curve area under the curve (ROC AUC) results are shown. Sensitivity and specificity were based on the 95th percentile of CM-5 in normal samples in each region (dotted line in histogram). Performance of individual markers from US samples is shown in Fig. S4. SCC—squamous cell carcinoma; CIN2/3—cervical intraepithelial neoplasia 2/3; CIN1—cervical intraepithelial neoplasia 1