Fig. 4
The effects of inhibiting histone H3K27 demethylases on the proliferation of cervical cancer cells. A The inhibitory effect of GSK-J4 on cell proliferation through the use of the CCK-8 Kit. B The results of colony formation assay in HeLa and SiHa cells treated with GSK-J4. C The immunofluorescence staining of Ki67, a marker of cell proliferation, using CLSM. The left image represents HeLa cells and the right image represents SiHa cells. The scale bar in the left image is 30 μm, and in the right image is 20 μm. D The results of Western blot analysis for Cyclin B1, CDK1, Bcl-2, Bax, caspase-7, caspase-3, and cleaved caspase-3 (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001 compared to the control group