Fig. 4
Effect of shDNMT1 on the self-renewal and tumor growth of H460-derived LCSLCs A DNMT1 activity; B DNMT1 mRNA and protein expression; C miR-152-3p levels; D miR-152-3p promoter methylation; E SOS1 mRNA and protein expression; F Rates of sphere and colony formation (scale bar, 100 μm); G, H Protein expression of CD44, CD133, Oct4, and Sox2. All experiments were conducted in triplicate. *p < 0.05 vs. NC; I Subcutaneous xenografts of LCSLCs (1 × 105) treated with shNC or shDNMT1; J Tumor volume and weight; K H&E staining and immunohistochemistry for the expression of DNMT1, SOS1, and CD44 proteins, and in situ immunofluorescence hybridization of miR-152-3p (scale bar, 50 μm). Data were obtained from xenografts from six mice per group. *p < 0.05 vs. NC