Fig. 6

HMAs have notable anti-ALL activity through the miR-182-PBX3/BCL2 axis in ALL cells. A miR-182 levels were measured in NALM-6, REH, RS4;11, and Jurkat cells treated with or without AZA (5 μM) and DAC (5 μM) for four days. B and C The CpG 3 methylation frequency was analyzed by bisulfite genomic sequencing in NALM-6 and Jurkat cells treated with or without DAC (5 μM) for four days. Each row of circles represents the sequence of an individual clone. The black and empty circles represent methylated and unmethylated CpG dinucleotides, respectively (Left). Shown are the statistical analysis of the percentage of methylation (Right). D The frequency of methylation at CpG 3 was analyzed by bisulfite genomic sequencing in one primary ALL sample treated with or without DAC (5 μM) for two days. Shown is the statistical analysis of the percentage of methylation (Right). E The protein expression levels of PBX3 and BCL2 were measured in NALM-6 and Jurkat cells incubated with or without AZA (5 μM) and DAC (5 μM) for four days. F–I Apoptosis was measured in NALM-6, RS4;11, REH, and Jurkat cells treated with or without AZA (5 μM) and DAC (5 μM) for four days. J miR-182 levels were measured in four primary ALL samples with miR-182 promoter hypermethylation and two with hypomethylation that were treated with DAC (5 μM) for two days. *P < 0.05; **P < 0.01; ***P < 0.001. NS: Not significant