Fig. 4

Identification of differentially expressed miR-20b-5p in plasma samples from ASD patients and 12-day cardiac-differentiated hESCs. A Volcano plot of differentially expressed miRNAs in plasma samples from ASD patients or healthy controls (n = 9–10). The red and green dots represented significantly upregulated and downregulated miRNAs in ASD group, respectively. B Schematic illustration of candidate miRNAs significantly upregulated in plasma samples from ASD patients but downregulated in 12-day cardiac-differentiated hESCs. C Heatmap of the eight candidate miRNAs upregulated in plasma samples from ASD patients (n = 9-10). D Heatmap of the eight candidate miRNAs downregulated during 12-day cardiac differentiation of hESCs (n = 3). E qRT-PCR analysis of miR-20b-5p expression level in plasma samples from ASD patients or healthy controls (n = 9). F qRT-PCR analysis of the relative TET2 mRNA expression level in plasma samples from ASD patients or healthy controls (n = 9). D0, D3, D6, D9 and D12 indicated the time points at Day 0, Day 3, Day 6, Day 9 and Day 12, respectively. Quantitative data were presented as mean ± SEM, while statistical significance was analyzed via a two-tailed unpaired Student’s t test and represented as *P < 0.05 and ***P < 0.001