Fig. 1

Establishment of hESCs-derived in vitro cardiac differentiation. A Scheme of in vitro cardiac differentiation derived from hESCs. B Immunofluorescence of cTnT after 12-day differentiation (Scale bar = 20 μm). C-G The relative mRNA expression levels of cardiac markers (GATA4, NKX2.5, TBX5, MYH6 and cTnT) during 12-day differentiation were tested by qRT-PCR (n = 3-4). H The relative protein levels of cardiac markers (GATA4, NKX2.5, TBX5, MYH6 and cTnT) during 12-day differentiation were detected by western blotting (n = 3). I Flow cytometry analysis of cTnT-positive cell during 12-day differentiation (n = 3). D0, D3, D6, D9 and D12 indicated the time points at Day 0, Day 3, Day 6, Day 9 and Day 12, respectively. Quantitative data were presented as mean ± SEM, while statistical significance was analyzed via a one-way ANOVA followed by Bonferroni multiple comparisons test and represented as *P < 0.05, **P < 0.01 and ***P < 0.001