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Fig. 4 | Clinical Epigenetics

Fig. 4

From: MAL expression downregulation through suppressive H3K27me3 marks at the promoter in HPV16-related cervical cancers is prognostically relevant and manifested by the interplay of novel MAL antisense long noncoding RNA AC103563.8, E7 oncoprotein and EZH2

Fig. 4

RNA immunoprecipitation (RIP)-qPCR with E7 and EZH2 antibody. A Percent input showing the enrichment of AC103563.8 in SiHa compared to that of IgG control upon E7 Immunoprecipitation. B Fold enrichment showing the enrichment of AC103563.8 in SiHa compared to that of IgG control upon E7 Immunoprecipitation. The data are represented as mean ± SD from two independent experiments. C Percent input showing the enrichment of AC103563.8 in transfected SiHa (T SiHa) compared to that of untransfected SiHa (UT SiHa) upon EZH2 Immunoprecipitation. D Fold enrichment showing the enrichment of AC103563.8 in transfected SiHa (T SiHa) compared to that of untransfected SiHa (UT SiHa) upon EZH2 Immunoprecipitation. NEAT1 is shown as the positive control for EZH2 interaction in UT and T SiHa. The data are represented as mean ± SD from two independent experiments

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Clinical Epigenetics

ISSN: 1868-7083

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