Fig. 2
From: Abnormal DNA methylation within HPA-axis genes years after paediatric critical illness
Interaction with age of abnormal DNA methylation in former PICU patients 2 years after critical illness vs healthy children. Methylation profiles are shown for each of the CpG sites that, adjusting for baseline risk factors and technical variation, showed a significant interaction between differential methylation in former PICU patients (n = 818) vs healthy children (n = 392) and ‘age at exposure’ (interaction p values obtained with multivariable linear regression analyses using the limma framework: cg03591753 p = 0.0060, cg03546163 p = 0.00063, cg06571187 p = 0.027, cg14139038 p = 0.0020). Univariate linear regression lines are drawn for methylation status of the CpG site in function of age at exposure, for former PICU patients (red) and healthy children (blue), where the shaded area represents the 95% confidence interval. Abbreviations: PICU: paediatric intensive care unit, UTR: untranslated region