Fig. 3

TCF-1 expression correlated with early differentiation state and hypo-effector function in human peripheral T cells. a–c Per-cell histone acetylation was stained together with TCF-1 and surface markers. a Representative dot-plots of acH3 versus TCF-1 (Left), and statistical analysis (N = 17) (right) of acH3 gMFI between TCF-1^hi and TCF-1^lo CD8⁺ (top) and CD4⁺ (CD3⁺CD8⁻) (bottom) T cells. acH3 gMFIs of TCF-1^hi and TCF-1^lo cells derived from the same donor were connected. b & c Histograms and summary results (N = 51) of TCF-1 expression in CD8⁺ (b) and CD4⁺ (CD3⁺CD8⁻) (c) T cell subsets. d & e ICS was performed the same as mentioned in Fig. 2d. d Representative images of effector cytokine production (left), TCF-1 expression in IFN-γ⁺ TNF-α⁺, IFN-γ⁺ TNF-α⁻ and IFN-γ⁻ TNF-α⁻ CD8⁺ T cells (middle), and a summary graph (N = 13) of TCF-1 gMFI in the three aforementioned T cell populations (right). e Representative dot-plots of IL-2 production versus TCF-1 expression (left) in CD8⁺ T cells, and statistical analysis (N = 13) on the frequency of IL-2⁺ cells in TCF-1⁺ and TCF-1⁻ subpopulations (right)