Fig. 2

Per-cell histone acetylation was enriched in terminally differentiated human peripheral T cell subsets. a–c. Single-cell histone acetylation was stained in combination with surface markers to make comparisons among individual T cell subsets, representative histograms (left) and violin plots (right) summarizing gMFI of acH3 (a), acH4 (b) in CD8⁺, or acH3 in CD4⁺ (CD3⁺CD8⁻), (c) T_N, T_EMRA, T_CM, T_EM, and T_SCM were shown. d PBMCs were stimulated with PMA + I for 4 h in the presence of brefeldin A, ICS was performed to detect cytokine production (left), representative histograms (middle) and statistical results (right) of acH3 in IFN-γ⁺ TNF-α⁺, IFN-γ⁺ TNF-α⁻ and IFN-γ⁻ TNF-α⁻ CD8⁺ T cells were presented. Summary results derived from 51 individuals in (a & c), 23 individuals in (b), and 13 independent experiments in (d). *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001