Fig. 3

ACTA2-AS1 inhibited papillary thyroid carcinoma (PTC) metastasis. A, B Transwell invasion assay for the invasive ability of ACTA2-AS1-overexpressing TPC1 cells (A) and the respective quantitative analysis (B). Scale bar, 50 µm and magnification, 200× . C, D Transwell invasion assay for the invasive ability of ACTA2-AS1-silenced BCPAP cells (C) and the respective quantitative analysis (D). Scale bar, 50 µm and magnification, 200× . E, F ACTA2-AS1-overexpressing TPC1 cells were injected into nude mice via the tail vein (n = 3) and the extent of tumor metastasis was determined after 9 weeks using in vivo bioluminescent imaging. G Hematoxylin and eosin (H&E) staining was carried out to assess cancer metastasis to lung tissues. Scale bar, 100 µm and magnification, 100× . H Quantification of lung metastatic nodules. *p < 0.05, **p < 0.01