Fig. 1

Sarcoma classifier results and histologic review of selected cases. A: Results of group comparisons based on DNAm profiling analysis with Sarcoma classifier. Group 1 comprises tumors with homogenous histology, malignant peripheral nerve sheath tumors (MPNST)/malignant triton tumor (MTT)—upper panel, and atypical neurofibroma (ANF)—lower panel, so the pie charts represent the different results of DNAm profiling. Conversely, samples of group 2 were selected because of a homogenous MPNST-like DNAm signature, and the donut chart represents the intra-group histological variability. B: Hematoxylin–eosin staining (left) and immunohistochemistry results for BCOR (right) confirming the diagnosis of BCOR-altered sarcoma suggested by DNAm profiling for case #11. C: Hematoxylin–eosin staining (left) and immunohistochemistry results for MyoD1 (right) confirming the diagnosis of RMS MYOD1-mutated suggested by DNAm profiling for case #12. Picture magnification is 20X (B and C, right panel) or 4X (C, left panel)