Fig. 4

Changes in the DNA Methylation Profile of the TSF Group. A The circus plot indicates the changes in the methylation status of specific methylation sites. The red region represents hyper-methylation, whereas the green region represents hypo-methylation; B Gene Ontology (GO) analysis illustrates a large proportion of DMGs (> 60%) involved in transcription regulation and molecular binding (protein, metal-ion, ATP, and DNA) processes; C The Bubble plot shows the associated signaling pathways enriched in highly methylated genes; D The overlap of the reported and research-wised methylation-related homologous genes. The green circle indicates down-regulated methylation genes by the TSF group versus the HM + STZ group. The blue circle stands for up-regulated methylation genes by the HM + STZ group versus the control. The pink circle represents the published methylation-related homologous genes associated with homocysteine