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Fig. 4 | Clinical Epigenetics

Fig. 4

From: TET2-mediated ECM1 hypomethylation promotes the neovascularization in active proliferative diabetic retinopathy

Fig. 4

Verification of expression and promoter methylation of the three key genes. A Migration assay of HRMECs. B Tube formation assay of HRMECs. C Quantitative analysis of promoter methylation level of the three key genes. D Protein levels of three key genes after TET2 knockdown. E Quantitative analysis of promoter methylation levels of the three key genes. F Agarose gel electrophoresis results of MS-PCR products of the key genes. MS-PCR, methylation-specific PCR; M, methylation bands; U, unmethylated bands; M/U, quantifying the ratio of the intensity of the methylated band to the unmethylated band; NC, normal control; HG, high glucose

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