Fig. 4
siSirt6 reverses the cardioprotective effect of S-ASC-Exo. A LDH measured by ELSIA (*P = 0.012, #P = 0.025). B Cell viability measured by MTT (*P = 0.014, #P = 0.033). C Representative western blot pictures showing expressions of Sirt6, AIM2, GSDMD, p62, and Beclin-1. D mRNA expressions of Sirt6 (*P = 0.024, #P = 0.031), AIM2 (*P = 0.04, #P = 0.045), GSDMD (*P = 0.015, #P = 0.035), p62 (*P = 0.029, #P = 0.036), and Beclin-1 (*P = 0.017, #P = 0.027). E Densitometric quantification of Sirt6 (*P = 0.031, #P = 0.038), AIM2 (*P = 0.001, #P = 0.013), GSDMD (* P = 0.007, #P < 0.001), p62 (*P = 0.021, #P = 0.029), and Beclin-1 (*P = 0.003, #P = 0.005) expressions. *P < 0.05, versus control, #P < 0.05, versus S-ASC-Exo