A targeted long-read sequencing approach questions the association of OXTR methylation with high-functioning autism

Table 3 Analysis of 5mC modification in regions or CpG sites frequently associated with differential methylation in ASD or with autism traits, social cognition alterations or social anxiety (Moerkerke et al., 2021) within our nanopore sequencing dataset. No significant differentially methylated loci were found in the oft-cited MT2 region within the CpG island or across exon 3, even at single CpG positions that previous studies have shown to be frequently altered in autism

Region	Hg38chr3:	bp	CpGs	5mC modification		diff	diff.se	stat	pval
				HFA	NC
MT2	8,769,033–8769438	405	26	0.156	0.153	0.003	0.022	0.132	0.630
Exon 3	8,768,329–8,767,266	1064	108	0.141	0.148	0.007	0.020	0.401	0.571
bp—TSS
− 860	8,769,047			0.247	0.209	0.038	0.032	1.194	0.232
− 901	8,769,088			0.677	0.660	0.017	0.037	0.462	0.644
− 924	8,769,111			0.517	0.512	0.005	0.037	0.148	0.882
− 934	8,769,121			0.517	0.512	0.005	0.037	0.148	0.882
− 959	8,769,146			0.368	0.381	0.013	0.036	-0.359	0.719
− 982	8,769,169			0.095	0.089	0.006	0.021	0.287	0.774
− 989	8,769,176			0.095	0.089	0.006	0.021	0.286	0.774

ISSN: 1868-7083