Table 3 Antibody used in the study
From: Histone acetylation: a key determinant of acquired cisplatin resistance in cancer
Protein | Company | Catalog number | Dilution | Purpose |
|---|---|---|---|---|
H3 | Sigma | H-0164 | 1:5000 | WB |
pAKT | Cell Signaling | 43966 | 1:1000 | WB |
pH2AX | Millipore | 05-636 | 1:5000 | WB |
H3K9ac | Millipore | 04-1003 | 1:5000 1:100 1:100 | WB IF IHC |
H3K27ac | abcam | ab4729 | 1:5000 2 µg/10 µg chromatin | WB ChIP |
H3K56ac | abcam | 76307 | 1:3000 | WB |
H4K5ac | Millipore | 06-759 | 1:5000 | WB |
H4K16ac | Millipore | 07-329 | 1:8000 2 µg/10 µg chromatin 1:100 | WB ChIP IF |
H3K9Me3 | abcam | 8898 | 1:4000 | WB |
Pan-acetyl lysin | abcam | ab80178 | 1:1000 | WB |
Secondary anti-rabbit | Cell Signaling | 7074 | 1:8000 | WB |
Secondary anti-mouse | Sigma | A-4416 | 1:5000 | WB |
Caspase 3 | Cell signaling | 9662 | 1:100 | IHC |
β-actin | Sigma | A-5316 | 1:10,000 | WB |