Fig. 2
From: Activation of γ-globin expression by LncRNA-mediated ERF promoter hypermethylation in β-thalassemia
LncRNA RP11-196G18.23 mediates ERF promoter hypermethylation and leads to reactivation of γ-globin. A The ERF promoter methylation level examined by clone-seq in HUDEP-2. Each row of eight CpG sites within a group represents a single bisulfite-treated clone with methylated CpGs (●) or unmethylated CpGs (○). B, C The ERF and γ-globin mRNA and protein levels were examined by qPCR (B) or Western blotting (C) in RP11-196G18.23 OE HUDEP-2 cells. The band intensities measured by ImageJ were showed underneath each panel. D The ERF promoter methylation level in CD34+ HSPCs. E, F The ERF protein level examined by Western blot (E) and the Hb F production examined by HPLC (F) in CD34+ HSPCs. G qPCR analysis of ERF and γ-globin mRNA level in wild type (Ctrl) and RP11-196G18.23 binding sequences disrupted HUDEP-2 cells. Data are shown as the means ± SEM from at least two independent experiments (*p < 0.05; ***p < 0.001)