Table 3 Validation of the methylation differences of CpGs between asthmatic subjects and controls in independent validation ACEs dataset
CpG | Gene | Effect size in discovery set (n = 96 asthmatics, n = 46 controls) | Effect size in validation set (n = 74 asthmatics, n = 41 controls) | Direction of association | ||
|---|---|---|---|---|---|---|
Δmeth (100%) | Adjusted P value | Δmeth (100%) | Adjusted P value | |||
cg00406211 | GRK5 | − 11.335 | 2.63E−06 | − 12.978 | 1.39E−09 | Hypo |
cg21385480 | LRIG1 | 7.7035 | 0.0002 | 9.3715 | 4.45E−09 | Hyper |
cg10528482 | SLC9A3 | 8.1986 | 0.0007 | 8.968 | 2.76E−05 | Hyper |
cg16393012 | ARHGDIB | − 5.627 | 0.010 | − 8.863 | 1.46E−05 | Hypo |
cg25267808 | MAML2 | 6.403 | 0.006 | 6.661 | 6.16E−05 | Hyper |
cg18181229 | PBX1 | 5.285 | 0.009 | 5.417 | 0.001 | Hyper |
cg14185918 | KLF4 | − 4.071 | 0.006 | − 5.009 | 0.0001 | Hypo |
cg10290200 | FLNC | − 2.633 | 0.001 | − 4.843 | 3.08E−06 | Hypo |
cg02766259 | AACS | − 4.119 | 0.011 | − 4.623 | 0.0004 | Hypo |
cg06128142 | GPT2 | 3.595 | 0.001 | 4.277 | 5.91E−06 | Hyper |
cg23817893 | CCDC81 | 4.435 | 0.003 | 4.1574 | 0.003 | Hyper |
cg01975495 | SERPINE1 | 4.669 | 0.003 | 3.989 | 0.002 | Hyper |
cg26639146 | CD9 | − 3.696 | 0.039 | − 3.709 | 0.009 | Hypo |
cg03441945 | ABAT | 3.402 | 0.014 | 3.573 | 0.001 | Hyper |
cg17602126 | HEYL | 3.188 | 0.002 | 3.307 | 0.001 | Hyper |
cg25477769 | HNF1A | 2.497 | 0.017 | 3.304 | 7.98E−05 | Hyper |
cg19212949 | PEG3 | − 1.943 | 0.027 | − 3.243 | 0.0001 | Hypo |
cg08801887 | TCIRG1 | 2.366 | 0.017 | 3.127 | 4.58E−05 | Hyper |
cg11419403 | CLPTM1L | − 2.66 | 0.009 | − 2.749 | 0.016 | Hypo |
cg15012607 | ETHE1 | 2.046 | 0.043 | 2.554 | 0.0005 | Hyper |
cg10672136 | TPO | 1.551 | 0.001 | 2.512 | 6.98E−08 | Hyper |
cg17945560 | TINAGL1 | 2.037 | 0.011 | 2.383 | 0.0032 | Hyper |
cg15562220 | SCGN | 1.964 | 0.018 | 2.329 | 0.006 | Hyper |
cg23556108 | BCL11A | 1.652 | 0.001 | 2.1769 | 0.0001 | Hyper |
cg19651003 | BSG | − 1.589 | 0.004 | − 2.164 | 1.44E−05 | Hypo |
cg10336131 | CNIH2 | 1.941 | 0.007 | 1.963 | 0.008 | Hyper |
cg20491914 | KCNK3 | 1.281 | 0.045 | 1.632 | 0.017 | Hyper |
cg09048665 | WDR90 | − 1.233 | 0.033 | − 1.412 | 0.012 | Hypo |
cg17012160 | FMN2 | 1.200 | 0.019 | 1.290 | 0.002 | Hyper |