Methylation of serotonin regulating genes in cord blood cells: association with maternal metabolic parameters and correlation with methylation in peripheral blood cells during childhood and adolescence

Table 2 Hierarchical linear regression analysis of maternal metabolic parameters as predictors of SLC6A4 methylation (mean of CpG sites #1 to #15) in cord blood cells

Step	Variable	B	SE (B)	p (B)	R²	p (R²)	ΔR²	p (ΔR²)
1	Neonatal sex	− 2.47	0.21	< 0.0001	0.416	< 0.0001
2	Neonatal sex	− 2.43	0.21	< 0.0001	0.422	< 0.0001	0.006	0.136
2	pBMI	− 0.02	0.02	0.136	0.422	< 0.0001	0.006	0.136
3	Neonatal sex	− 2.39	0.21	< 0.0001	0.441	< 0.0001	0.019	0.010
	pBMI	− 0.04	0.02	0.030
	GWG	− 0.05	0.02	0.010
4	Neonatal sex	− 2.39	0.21	< 0.0001	0.442	< 0.0001	< 0.001	0.705
	pBMI	− 0.04	0.02	0.028
	GWG	− 0.05	0.02	0.014
	GTS	0.08	0.22	0.705
5	Neonatal sex	− 2.38	0.21	< 0.0001	0.459	< 0.0001	0.018	0.100
	pBMI	− 0.04	0.02	0.031
	GWG	− 0.06	0.02	0.004
	GTS	0.01	0.22	0.952
	Parity	− 0.42	0.22	0.054
	Maternal age	0.02	0.02	0.280
	Birth weight	0.00	0.00	0.223

B—unstandardized beta coefficient; SE (B)—standard error of B; p (B)—significance for each of the predictors; R²—proportion of variance explained by predictors; p (R²)—significance of R²; ΔR²—increase in R² resulting from the addition of predictor/s; p (ΔR²)—significance of ΔR²; pBMI—pre-pregnancy body mass index; GWG—gestational weight gain; GTS—glucose tolerance status (NGT vs. GDM, NGT as a reference). Significant p values are in bold

ISSN: 1868-7083