Fig. 6

KDM4B interacts with MYC to promote cell proliferation for epigenetic activation. A Wild-type and mutant KDM4B plasmids with Flag tags were constructed. The KDM4B mutant contained multiple point mutations in the JmjC region. B Plate colony formation assays were used to evaluate the colony formation ability of GBM cells after restoration of wild-type KDM4B or mutant KDM4B expression. C MTT assays were used to determine the proliferative capacity of GBM cells after restoration of wild-type KDM4B or mutant KDM4B. D Western blots were applied to evaluate the levels of Flag, FBXL3, CRY2, MYC, cyclin B1, GAPDH, H3K9me3, and H3 after restoration of wild-type KDM4B or mutant KDM4B. E IP assays were used to detect the interaction between KDM4B and C-MYC in GBM cells. F ChIP assays were used to detect the binding of KDM4B to the promoter of CCNB1. G Schematic diagram of the mechanism by which KDM4B participates in the regulation of CCNB1. *p < 0.05, **p < 0.01, ***p < 0.001