Fig. 4

KDM4B silencing prevents cancer cell survival, proliferation, migration, and invasion through the MYC signaling pathway. A Flow cytometry analyses of the cell cycle in GBM cells with KDM4B knockdown. B Heatmap showing differential gene expression in U87-MG/shGFP or shKDM4B cells. C Correlation analysis of CCNB1 with KDM4B was performed using the GEPIA database. D Western blots were applied to check the protein levels of KDM4B, MYC, p27, cyclin B1, CDK1, tubulin, H3K9me3, and H3 in KDM4B knockdown cells. E Transcriptome data were used to analyze MYC signaling pathway changes through GSEA V4.2.3. The gene set was downloaded from GSEA's official website (https://www.gsea-msigdb.org/gsea/msigdb/ cards/ALFANO_MYC_TARGETS). F-G Correlation analysis of MYC and CDH1 with KDM4B was performed using the GEPIA database. H Western blots were applied to check the protein levels of Snail, Slug, E-cadherin, MMP2, and α-Tubulin in KDM4B knockdown cells