Fig. 6
From: EZH2 K63-polyubiquitination affecting migration in extranodal natural killer/T-cell lymphoma
HSP60 is an upstream modulator of TRIP12-EZH2 signaling. A Co-IP showing interaction between HSP60 and EZH2 in YT (left) and NKYS (right) cells. B Co-IP showing interaction between HSP60 and SUZ12 in YT (left) and NKYS (right) cells. C Immunoblots indicating decrease of both TRIP12 and EZH2 with siRNA-mediated knockdown of HSP60 in NK92 cells. D Change of TRIP12 and EZH2 mRNA level with TRIP12 knockdown in YT (upper) and NKYS (lower) cells. E Change of interaction between HSP60 and EZH2 or its mutants with TRIP12 overexpression in HEK293T cells. N = 3 individual experiments and representative images are shown