Fig. 5

TRIP12 mediates EZH2 K634 ubiquitination and meanwhile enhances PRC2 stability and T487 phosphorylation. A Change of exogenously overexpressed EZH2 and endogenous p-EZH2 (T487) level with indicated transfections. B Co-IP showing change of interaction between TRIP12 and EZH2 mutant. C Schematic diagram indication: the conservation of EZH2 K634. (D) Co-IP showing change of interaction between EZH2 and PRC2 components or histone H3 with TRIP12 overexpression. E Co-IP showing change of interaction between EZH2 and CDK1 with or without TRIP12 overexpression. F Co-IP showing change of interaction between CDK1 and EZH2 mutant with TRIP12 overexpression. G Co-IP showing change of interaction between EZH2 or its mutants and SUZ12 with TRIP12 overexpression. All immunoblots or co-IPs were performed in HEK293T cells, and the cells were harvested for lysis 48 h after transfection. N = 3 individual experiments and representative images are shown