Fig. 1

E3 ligase TRIP12 correlates with EZH2 expression in extranodal natural killer/T-cell lymphoma (ENKTL). A Gene expression profiling (GEP) data showing EZH2 expression in ENKTL: 35 patient samples and 6 normal control tissues. B Co-IP showing interaction between EZH2 and TRIP12 or UHRF1 in YT (left) and NK92 cells (right). C EZH2 protein level change upon TRIP12 knockdown using siRNA in YT (upper) and NKYS (lower) cells. N = 3 individual experiments and representative images are shown. ENKTL cells were harvested for immunoblots 48 h after knockdown with Neon transfection system. D EZH2 mRNA level change upon TRIP12 knockdown using siRNA in YT (upper) and NKYS (lower) cells. ENKTL cells were harvested for RNA extraction 24 h after knock down. E EZH2 mRNA level change upon Flag-TRIP12 or empty vector transfection in YT (upper) and NKYS (lower) cells. The cells were harvested for RNA extraction 16 h after overexpression. Results are mean ± SD. N = 3; N.S. not significant; *p < 0.05; **p < 0.01; ***p < 0.001. F linear correlation between TRIP12 and EZH2 expression obtained from GEP data of 35 ENKTL patient samples (R = 0.76, p = 6.9e−07)