Fig. 2

A Average percentage of CpGs methylation values within ZEB1 gene in cell lines by increasing order, detected by pyrosequencing (PyroSeq). B ZEB1 expression in unmethylated (U) and highly methylated (M) paired normal tissues-tumors (N–T) (n = 10 for each group), calculated by 2^−ΔCt method. C Negative ZEB1 staining (methylated tumor), including positive nuclear staining in fibroblasts as internal control (up); positive nuclear ZEB1 staining in tumoral cells (unmethylated tumor) (down) (magnification: ×400). D Restoration of ZEB1 expression in HCT116 and HT29 cells by treatment of control cells (Ctl) with 5-aza-dC (AZA), trichostatin (TSA) and AZA + TSA, as calculated by 2^−ΔΔCt method (* p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001)